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- 详细信息
- 询价记录
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- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 品系：
129S2/SvPas
- 细胞类型：
其他细胞类型
- 生长状态：
贴壁生长
- 库存：
大量
- ATCC Number：
CRL-1934™
- 细胞形态：
球形
- 器官来源：
胚胎
- 运输方式：
冻存运输
- 年限：
embryo, blastocyst
| Designations: | ES-D3 [D3] | ||
| Depositors: | T Doetschman | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | spherical colony |
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| Source: | Organ: embryo Strain: 129S2/SvPas Cell Type: pluripotent embryonic stem cell; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | Undifferentiated cells can be genetically modified by gene targeting techniques. The cells spontaneously differentiate into embryonic structures in the absence of a feeder layer or conditioned medium. The clonal embryonic stem cell line ES-D3 was derived from blastocysts of a 129S2/SvPas mouse. Fibroblast-like feeder layer cells are present in the ampules sent by ATCC . |
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| Age: | embryo, blastocyst | ||
| Comments: | The clonal embryonic stem cell line ES-D3 was derived from blastocysts of a 129S2/SvPas mouse. Undifferentiated cells can be genetically modified by gene targeting techniques.The cells spontaneously differentiate into embryonic structures in the absence of a feeder layer or conditioned medium. They can be maintained in the undifferentiated state by frequent subculture (every 2 to 3 days) on confluent feeder layers (STO cells) irradiated at 12,000 Rads (see ATCC 56-X; irradiated STO cells). These ES-D3 cells are not germline competent. Fibroblast-like feeder layer cells are present in the ampules sent by ATCC . | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.1 mM ; fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C Growth Conditions: To maintain the cells in the undifferentiated state they must be grown on confluent feeder layers of irradiated STO cells (see 56-X, irradiated STO cells). |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 feeder layer cells:ATCC 56-X derivative:ATCC SCRC-1003 |
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| References: | 22394: Doetschman TC, et al. The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J. Embryol. Exp. Morphol. 87: 27-45, 1985. PubMed: 3897439 22928: Williams RL, et al. Myeloid leukaemia inhibitory factor maintains the developmental potential of embryonic stem cells. Nature 336: 684-687, 1988. PubMed: 3143916 23296: Gossler A, et al. Transgenesis by means of blastocyst-derived embryonic stem cell lines. Proc. Natl. Acad. Sci. USA 83: 9065-9069, 1986. PubMed: 3024164 23307: Doetschman T, et al. Targeted mutation of the Hprt gene in mouse embryonic stem cells. Proc. Natl. Acad. Sci. USA 85: 8583-8587, 1988. PubMed: 3186749 |
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文献和实验相关实验
人25羟维生素D3（25(OH)D3）酶联免疫分析（ELISA）
人 25羟维生素D3（25(OH)D3） 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中25羟维生素D3（25(OH)D3）含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 人25 羟维生素 D3 （ 25(OH)D3 ） 水平。用纯化的 人25 羟维生素 D3 （ 25(OH)D3 ） 抗体包被微孔板，制成固相抗体，往包
vitamin D3 analogs, it is reasonable to target the screening methods on the steps mentioned above.
小鼠维生素D3 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定小鼠血清，血浆及相关液体样本中 维生素D3 的 含量。 实验原理 ： 本试剂盒应用 双抗体 夹心法测定 标本 中 小鼠 维生素D3 水平。用纯化的 小鼠 维生素D3 抗体 包被微孔板，制成固相 抗 体，往包被 单抗 的微孔中加入 维生素D3 ，再与HRP 标记的 维生素D3 抗体结合，形成抗体- 抗原
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ES-D3 [D3]
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