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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 细胞形态:
淋巴样
- 运输方式:
冻存运输
- 物种来源:
人
- 是否是肿瘤细胞:
1
- 细胞类型:
T淋巴细胞
- ATCC Number:
CRL-8543™
- 库存:
大量
- 生长状态:
悬浮生长
| Designations: | H9/HTLV-IIIB | ||
| Depositors: | The United States of America | ||
| Biosafety Level: | 2 [Cells Contain RETROVIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: T lymphocyte; | ||
| Cellular Products: | Human T cell leukemia virus III (HTLV III); Human Immunodeficiency virus (HIV) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | The cells produce HTLV III. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| References: | 3536: Popovic M, et al. Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions. US Patent 4,520,113 dated May 28 1985 22938: Chang SY, et al. The origin of HIV-1 isolate HTLV-IIIB. Nature 363: 466-469, 1993. PubMed: 8502298 32754: Trkola A, et al. Human momoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. J. Virol. 70: 1100-1108, 1996. PubMed: 8551569 |
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文献和实验ES-D3 CRL-1934 小鼠 多能胚胎干细胞 胚胎型 DMEM+ 15% FBS F9 CRL-1720 小鼠 睾丸畸胎癌 胚胎型至内胚层 DMEM, 15% FBS GH1 CCL-82 大鼠 垂体肿瘤 上皮细胞、贴壁 F-12, 15% HS和2.5% FBS GH3 CCL-82.1 大鼠 垂体肿瘤 上皮细胞、贴壁 F-12, 15% HS和2.5% FBS H9 HTB-176 人 T细胞淋巴瘤 成淋巴细胞、悬浮 RPMI-1640, 10% FBS H9/HTLV
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