Hs 454.T

Hs 454.T

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  • 询价
  • 2025年08月11日
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    • 详细信息
    • 技术资料
    • 生长状态

      贴壁生长

    • 年限

      2 years

    • 细胞形态

      成纤维样

    • 相关疾病

      其他疾病

    • 器官来源

    • ATCC Number

      CRL-7802™

    • 物种来源

    • 是否是肿瘤细胞

      1

    • 运输方式

      瓶装运输

    • 库存

      大量

    Designations: Hs 454.T
    Biosafety Level: 1
    Shipped: flask
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens
    Morphology: fibroblast

    Source: Organ: bone
    Disease: eosinophilic granuloma
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Age: 2 years
    Gender: male
    Ethnicity: Caucasian
    Comments: Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
    Please see the NBL Repository description.
    Note: This item is distributed only within the 50 United States. It is not available for international distribution.
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0°C
    Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
    Medium Renewal: Every 2 to 3 days
    Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
    Add fresh culture medium, aspirate and dispense into new culture flasks.
    Preservation: culture medium 95%; DMSO, 5%
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
    recommended serum:ATCC 30-2020

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