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- 详细信息
- 文献和实验
- 技术资料
- 生长状态：
贴壁生长
- 年限：
2 years
- 细胞形态：
成纤维样
- 相关疾病：
其他疾病
- 器官来源：
骨
- ATCC Number：
CRL-7802™
- 物种来源：
人
- 是否是肿瘤细胞：
1
- 运输方式：
瓶装运输
- 库存：
大量
| Designations: | Hs 454.T | ||
| Biosafety Level: | 1 | ||
| Shipped: | flask | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | fibroblast |
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| Source: | Organ: bone Disease: eosinophilic granuloma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | 2 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage. Please see the NBL Repository description. Note: This item is distributed only within the 50 United States. It is not available for international distribution. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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文献和实验454测序仪的出现极大促进了测序业务的开展，科研人员已经将测序技术作为解决科研工作中许多常见 问题的利器。这是因为454测序仪在以下几个方面取得了质的突破：首先是解决了高通量测序问题；其次它简 化了样品准备步骤，将以往转化大肠杆菌扩增质粒的繁琐过程全部用简单的体外PCR扩增法替代了；最后， 它缩小了测序反应体积，节省了 试剂 。这样，454测序仪做到了以极其低廉的价格进行大规模平行测序反应。 它的测序规模之大、测序费用之低
猴 B病毒 (HS) 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定猴血清，血浆及相关液体样本中 B病毒(HS) 的 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 猴 B病毒 (HS) 水平。用纯化的 猴 B病毒 (HS) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 B病毒 (HS) ， 再与HRP 标记的 B 病毒 (HS) 抗体结合，形成
454 Life Sciences is a biotechnology company based in Branford, Connecticut specializing in high-throughput DNA sequencing using a novel massively parallel sequencing-by-synthesis approach. 454 has experienced rapid growth since its partnership
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