FHs 74 Int

FHs 74 Int

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  • 2025年09月14日
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    • 详细信息
    • 询价记录
    • 技术资料
    • 细胞形态

      上皮样

    • 生长状态

      贴壁生长

    • 相关疾病

      正常

    • ATCC Number

      CCL-241™

    • 库存

      大量

    • 器官来源

      小肠

    • 年限

      3 to 4 months gestation

    • 物种来源

    • 是否是肿瘤细胞

      0

    • 运输方式

      冻存运输

    Designations: FHs 74 Int
    Depositors:  R Owens
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens
    Morphology: epithelial

    Source: Organ: small intestine
    Disease: normal
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Receptors: epidermal growth factor (EGF), expressed
    Tumorigenic: No
    DNA Profile (STR): Amelogenin: X
    CSF1PO: 10,11
    D13S317: 11,12
    D16S539: 11,12
    D5S818: 11,12
    D7S820: 9,10
    THO1: 7,9.3
    TPOX: 8
    vWA: 16,18
    Cytogenetic Analysis: normal female; diploid
    Isoenzymes: AK-1, 1
    ES-D, 1-2
    G6PD, B
    GLO-I, 1
    Me-2, 1-2
    PGM1, 1
    PGM3, 1
    Age: 3 to 4 months gestation
    Gender: female
    Comments: PAS, negative.
    The cells are negative for keratin by immunoperoxidase staining.
    Propagation: ATCC complete growth medium: Hybri-Care Medium ATCC 46-X supplemented with 30 ng/ml epidermal growth factor (EGF), 90%; fetal bovine serum, 10%
    (Do not filter the medium after addition of EGF)
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Subculturing: Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. Add appropriate aliquots of the cell suspension to new culture vessels.
    6. Incubate cultures at 37�C.

        Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
        Medium Renewal: Every 2 to 3 days
    Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
    Storage temperature: liquid nitrogen vapor phase
    Related Products: recommended serum:ATCC 30-2020
    Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
    References: 22534: Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412
    22543: Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258
    26058: Smith HS, et al. Nuclear ultrastructure of epithelial cell lines derived from human carcinomas and nonmalignant tissues. Cancer Res. 39: 332-334, 1979. PubMed: 761205

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