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- 2025年09月14日
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- 细胞形态：
上皮样
- 生长状态：
贴壁生长
- 相关疾病：
正常
- ATCC Number：
CCL-241™
- 库存：
大量
- 器官来源：
小肠
- 年限：
3 to 4 months gestation
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
| Designations: | FHs 74 Int | ||
| Depositors: | R Owens | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: small intestine Disease: normal |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Receptors: | epidermal growth factor (EGF), expressed | ||
| Tumorigenic: | No | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 11,12 D5S818: 11,12 D7S820: 9,10 THO1: 7,9.3 TPOX: 8 vWA: 16,18 |
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| Cytogenetic Analysis: | normal female; diploid | ||
| Isoenzymes: | AK-1, 1 ES-D, 1-2 G6PD, B GLO-I, 1 Me-2, 1-2 PGM1, 1 PGM3, 1 |
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| Age: | 3 to 4 months gestation | ||
| Gender: | female | ||
| Comments: | PAS, negative. The cells are negative for keratin by immunoperoxidase staining. |
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| Propagation: | ATCC complete growth medium: Hybri-Care Medium ATCC 46-X supplemented with 30 ng/ml epidermal growth factor (EGF), 90%; fetal bovine serum, 10% (Do not filter the medium after addition of EGF) Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
|
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | recommended serum:ATCC 30-2020 Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X |
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| References: | 22534: Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412 22543: Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258 26058: Smith HS, et al. Nuclear ultrastructure of epithelial cell lines derived from human carcinomas and nonmalignant tissues. Cancer Res. 39: 332-334, 1979. PubMed: 761205 |
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