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- 文献和实验
- 技术资料
- 运输方式:
冻存运输
- 物种来源:
小鼠
- 是否是肿瘤细胞:
0
- 库存:
大量
- 免疫类型:
IgG2b
- 细胞形态:
淋巴样
- 生长状态:
悬浮生长
- ATCC Number:
CRL-2198™
| Designations: | IgG-7D4 | ||
| Depositors: | JL Goldstein, YK Ho | ||
| Isotype: | IgG2b | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against hamster sterol regulatory element binding protein 2 (SREBP 2, SREBP-2) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| Comments: | Animals were immunized with a bacterially produced fusion protein encoding 6 consecutive histidines followed by amino acids 32 to 250 of hamster SREBP. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody recognizes both the precursor and mature forms of hamster SREBP-2. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 23569: Yang J, et al. Three different rearrangements in a single intron truncate sterol regulatory element binding protein-2 and produce sterol-resistant phenotype in three cell lines. Role of introns in protein evolution. J. Biol. Chem. 270: 12152-12161, 1995. PubMed: 7744865 | ||
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