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- 免疫类型：
IgG1
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
- 库存：
大量
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 生长状态：
悬浮生长
- ATCC Number：
CRL-8016™
| Designations: | OKT 5 | ||
| Depositors: | Ortho Pharmaceutical Corporation | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human T cell (human cytotoxic/suppressor T lymphocyte) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with human peripheral blood T lymphocytes. Spleen cells were fused with P3X63Ag8.U1 myeloma cells. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium: Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | recommended serum:ATCC 30-2020 Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X |
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| References: | 3501: Kung PC, Goldstein G. Monoclonal antibody to human cytotoxic and suppressor T cells and methods of preparing same. US Patent 4,364,932 dated Dec 21 1982 | ||
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文献和实验Nature 综述导读：FDA 批准第 100 个抗体药物！抗体药物的研究方向在哪里？
Nature Reviews Drug Discovery 20, 10 (2021)单克隆抗体 (mAbs) 由 B 细胞产生，特异性靶向抗原。该产品能够得以大量生产制备归功于 Köhler 和 Milstein 于 1975 年发明的杂交瘤技术，该技术极大地提高了基础研究和临床应用的潜力，利用该技术，Schlossman 和他的合作者鉴定出一种针对特殊 T 细胞抗原的单克隆抗体 - OKT3，它针对的抗原便是 CD3。1981 年，他们在临床测试 OKT3 作为预防移植排斥的免疫抑制剂。1986 年
CD27 分子 CD27 常用单克隆抗体或代号： VIT14,S152,OKT18A 主要表达细胞： T, Bsub [T] 分子质量(kDa)和结构： p55(TNFR- SF)(同源二聚体) 功 能： CD70的配体,T细胞活化增殖 CD27 Marker of T cell activation CD27/CD70 interactions also regulate
: 由此步骤开始，流出的溶液包含目标细胞，请使用新的试剂管收集！首先，加入 1 ml 的 biotin 洗脱液，孵育 2 min。其次，加入 10 ml biotin 洗脱液，洗脱目标细胞。d) 再加入 10 ml biotin 洗脱液，洗下剩余的目标细胞。最后两步骤所收集的液体含分选出的目标细胞。(4) 结果分析以下流式图为从 7.5 ml 全血中，选出 CD3+ T 细胞的数据。选后细胞以 CD3-APC（OKT-3） 及 CD45-PE（HI30，白细胞标志） 抗体染色后，进行流式分析，死细胞
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