immunoglobulin; monoclonal antibody; against human C reactive protein
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Comments:
Animals were immunized with purified human C reactive protein. Lymph node cells were fused with P3X63Ag8.653 myeloma cells. Tested and found negative for ectromelia virus (mousepox). A feeder layer of mouse peritoneal macrophages or X-irradiated STO cells (ATCC 56-X) is helpful in the initiation of a culture from a thawed ampule.
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.