- 询价
- ATCC
- a'b01589
- 2025年09月17日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 生长状态：
悬浮生长
- 库存：
大量
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 免疫类型：
IgG2a; kappa light chain
- 细胞形态：
淋巴样
- ATCC Number：
HB-86™
- 运输方式：
冻存运输
| Designations: | HD2-4 | ||
| Depositors: | JF Kearney | ||
| Isotype: | IgG2a; kappa light chain | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human C reactive protein | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with purified human C reactive protein. Lymph node cells were fused with P3X63Ag8.653 myeloma cells. Tested and found negative for ectromelia virus (mousepox). A feeder layer of mouse peritoneal macrophages or X-irradiated STO cells (ATCC 56-X) is helpful in the initiation of a culture from a thawed ampule. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. | ||
| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Related Products: | feeder layer cells:ATCC 56-X | ||
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文献和实验Comprehensive High‐Throughput Arrays for Relative Methylation (CHARM)
5 ) CHARM HD2 microarrays and supplies for HD2 array hybridization and processing (NimbleGen): see the NimbleGen Array User's Guide at the URL above R Software (http://www
氏病(HD) HD是一种特殊类型的恶性淋巴瘤，组织学诊断主要依靠在多形性炎症浸润背景上找到特征性R-S细胞。1965年，Rye国际会议确定分为四型： 1．淋巴细胞为主型(LP)HD2以中、小淋巴细胞增生为主，有时以组织细胞增生为主；典型R-S细胞不易找到。但常存在较多L-H细胞。 2．结节硬化型(NS)HD：以双折光宽胶原纤维束，将存在腔隙型R-S细胞的淋巴组织分隔成大小不一结节为特征，典型R-S细胞罕见。 3．混合细胞型(MC)HD：典型R-S细胞
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