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- 文献和实验
- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 年限：
100 days
- ATCC Number：
CRL-10225™
- 库存：
大量
- 细胞形态：
成纤维样
- 运输方式：
冻存运输
- 组织来源：
subcutaneous connective tissue
- 品系：
C3H/An
- 生长状态：
贴壁生长
| Designations: | A9 L hD2 S.C. 18 [A9 L cell line hD2 subclone #18] | ||
| Depositors: | Receptor Genetics, Inc. | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus deposited as mouse | ||
| Morphology: | fibroblast |
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| Source: | Tissue: subcutaneous connective tissue Strain: C3H/An |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Receptors: | human dopamine D2 | ||
| Age: | 100 days | ||
| Gender: | male | ||
| Comments: | The cell line was derived from A9 L cells which are a derivative of L cells. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 46964: Brann MR, Stormann TM. Cloned genes for human dopamine D2 receptors and cell lines expressing same. US Patent 5,554,500 dated Sep 10 1996 | ||
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
, the cell pellet can be snap-frozen in liquid nitrogen and stored at -80°C until use. Restriction Enzyme Digestion and Intramolecular Ligation 12. Resuspend the pellet in 500 µL of 1.14X restriction enzyme buffer (appropriate
1 fibers in immunofluorescence and with rat type 1 MHCs (myosin heavy chain)in Western blotting;the cell line was established in 1986 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse that had been immunized with purified myosin
睡眠不足，免疫遭罪！最新 Cell 子刊：睡眠小于 6 小时，影响免疫应答，降低疫苗保护效果
and inflammation: partners in sickness and in health. Nat. Rev. Immunol. 19, 702–715. 3. Flanagan, K.L., Fink, A.L., Plebanski, M., and Klein, S.L. (2017). Sex and Gender Differences in the Outcomes of Vaccination over the Life Course. Annu. Rev. Cell Dev. Biol. 33
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