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- 详细信息
- 技术资料
- 免疫类型：
IgG2a
- 细胞形态：
淋巴样
- ATCC Number：
CRL-1943™
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- 生长状态：
悬浮生长
- 运输方式：
冻存运输
| Designations: | C44 | ||
| Depositors: | AC Cunningham | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against colchicine | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | The C44 hybridoma was originated by S.K. Edmond Rouan in 1987. Animals were immunized with a colchicine - keyhole limpet hemocyanin (KLH) conjugate. Spleen cells were fused with NS-1 myeloma cells. The antibody binds with high affinity to colchicine, and with lower affinity to related compounds. The antibody is bound strongly by Protein A. It is useful for monitoring therapeutic drug levels and has the potential to reverse colchicine toxicity. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: 2 to 3 times per week Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| References: | 22489: Edmond Rouan SK, et al. Specific, high affinity colchicine binding monoclonal antibodies: development and characterization of the antibodies. Hybridoma 8: 435-448, 1989. PubMed: 2777275 23185: Rouan SK, et al. Reversal of colchicine-induced mitotic arrest in Chinese hamster cells with a colchicine-specific monoclonal antibody. Am. J. Pathol. 137: 779-787, 1990. PubMed: 2221012 |
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C44
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