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- 生长状态：
悬浮生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 库存：
大量
- ATCC Number：
HB-9326™
- 免疫类型：
IgG2a
- 细胞形态：
淋巴样
| Designations: | ME361-S2a [ME361S2a] | ||
| Depositors: | Wistar Institute | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against GD2 and GD3 gangliosides | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with the SK MEL 23 human melanoma cells line. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with GD2 and GD3 gangliosides. It reacts preferentially with the GD2 and is largely unreactive with other gangliosides. It may be useful for diagnosis and therapy of human melanoma. This line is an isotype switch variant of ME361 (see ATCC HB-9325 ). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | culture medium, 85%; fetal bovine serum, 10%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 21930: Thurin J, et al. Monoclonal antibodies against melanoma-associated antigens and hybrid cell lines producing these antibodies. US Patent 4,849,509 dated Jul 18 1989 22351: Thurin J, et al. GD2 ganglioside biosynthesis is a distinct biochemical event in human melanoma tumor progression. FEBS Lett. 208: 17-22, 1986. PubMed: 3533633 |
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