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- 文献和实验
- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 生长状态：
贴壁生长
- 细胞类型：
其他细胞类型
- 运输方式：
冻存运输
- ATCC Number：
CRL-2498™
- 库存：
大量
- 细胞形态：
成纤维样
- 器官来源：
胚胎
- 年限：
9.5 days gestation
| Designations: | SYF + c-Src | ||
| Depositors: | RA Klinghoffer, P Soriano | ||
| Biosafety Level: | 2 [Cells contain Papovavirus; SV40 viral sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus deposited as mouse | ||
| Morphology: | fibroblast |
||
| Source: | Organ: embryo Cell Type: fibroblastimmortalized with SV40 large T antigen |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | The line is available with the following restrictions:1. This cell line was deposited at the ATCC by Dr. P Soriano and Dr. RA Klinghoffer. The cell line is provided for research purposesonly and is available under the condition that the material will not be used for commercial purposes or distributed it to third parties The cells are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the Fred Hutchinson Cancer Research Center,1100 Fairview Avenue North, C2M-027M, Seattle, WA 98109. Telephone (206) 667-6304, FAX (206) 667-4732. | ||
| Age: | 9.5 days gestation | ||
| Comments: | SYF (deficient for Src, Yes, and Fyn) cells were generated in 1997 from mouse embryos harboring functional null mutations in both alleles of the Src family protein tyrosine kinases, Src, Yes, and Fyn. The cells were immortalized before passage 2 by infection with a retroviral vector transducing the SV40 large T antigen. Cells deficient for Src, Yes, and Fyn are available as SYF (ATCC CRL-2459 ). c-Src was reintroduced into a SYF triple mutant cell line via the retroviral vector pLXSH. The SYF + c-Src cell line expresses wild type c-Src. The cells are both neomycin and hygromycin resistant. SYF + c-Src can be used as a control for SYF cells. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended Medium Renewal: Every 2 to 3 days |
||
| Preservation: | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101 Cell culture tested DMSO:ATCC 4-X |
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| References: | 38978: Klinghoffer RA, et al. Src family kinases are required for integrin but not PDGFR signal transduction. EMBO J. 18: 2459-2471, 1999. PubMed: 10228160 | ||
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