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- 2025年11月22日
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- 细胞形态：
成纤维样
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 器官来源：
胚胎
- 库存：
大量
- ATCC Number：
CRL-2205™
- 年限：
embryo
- 品系：
NIH/Swiss
| Designations: | PA317 LXSN 16E7 | ||
| Depositors: | DA Galloway | ||
| Biosafety Level: | 2 [Cells contain human papilloma viral DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
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| Source: | Organ: embryo Strain: NIH/Swiss |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Restrictions: | Note: The user of the PA317 LXSN 16E7 cell line agrees to indemnify and hold harmless the United States, the ATCC , Denise A. Galloway and Fred Hutchinson Cancer Research Center, Seattle, Washington from any claims, costs, damages, or expenses resulting from any injury (including death), damage or loss that may arise from the use of the cell line either directly (including use for diagnostic purposes) or in the preparation of a product. The user assumes all risks and responsibilities in connection with the receipt, handling, storage and use of the material. | ||
| Age: | embryo | ||
| Comments: | PA317 LXSN 16E7 is a packaging cell line developed by transfection of the retrovirus vector pLXSN16E7 into the Psi-2 ecotropic packaging cell line. Virions produced from the transfected Psi-2 cells were used to infect the amphotropic packaging line PA317, and infected cells were selected in medium containing G418. The pLXSN16E7 vector contains the human papilloma virus (HPV) type 16 E7 gene under control of the Moloney murine leukemia virus (MoMuLV) promoter- enhancer sequences. The vector also contains a gene controlling resistance to neomycin transcribed from the SV40 promoter. This line produces the amphotropic retrovirus LXSN16E6 which encodes the HPV16 E7 open reading frame, and which can be used to stably infect many cell types. The resulting cells constitutively express the E6 protein of HPV16 which binds to and inactivates the retinoblastoma (Rb) gene. The virus can be used to immortalize human keratinocytes. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended Medium Renewal: Every 2 to 3 days Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and remove trypsin, Add an additional 1 to 2 ml of trypsin solution, and allow the flask to sit at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 22566: Halbert CL, et al. The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. J. Virol. 65: 473-478, 1991. PubMed: 1845902 | ||
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文献和实验人 前白蛋白(PA) 酶联免疫分析 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，组织，细胞上清及相关液体样本中前白蛋白(PA) 的 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中人前白蛋白 (PA) 水平。用纯化的人前白蛋白 (PA) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入前白蛋白 (PA) ， 再与 HRP 标记的前白蛋白 (PA) 抗体
PROTOCOL TO EXTRACT DNA FROM PA
concentration is from 200-500μg/ml. 16.Denature the DNA at 70℃ before running 0.2μg on 1% gel to check the size. Size should range from 100bp-3Kb. Solutions Digestion buffer : 100mM NaCl/10mM Tris-HCl, pH 8.0 and 25mM EDTA, pH
PROTOCOL TO EXTRACT DNA FROM PA
-500μg/ml. 16.Denature the DNA at 70℃ before running 0.2μg on 1% gel to check the size. Size should range from 100bp-3Kb. Solutions Digestion buffer : 100mM NaCl/10mM Tris-HCl, pH 8.0 and 25mM EDTA, pH 8.0/0.5% SDS. Store at RT. Proteinase K: Stored
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