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- 详细信息
- 技术资料
- 相关疾病：
正常
- ATCC Number：
CRL-9483™
- 细胞形态：
上皮样
- 库存：
大量
- 运输方式：
冻存运输
- 组织来源：
bronchus
- 器官来源：
肺
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 生长状态：
贴壁生长
- 细胞类型：
其他细胞类型
| Designations: | BZR | ||
| Depositors: | The United States of America | ||
| Biosafety Level: | 2 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: lung Tissue: bronchus Disease: normal Cell Type: epithelialvirus transformed |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: XY CSF1PO: 9, 12 D13S317: 13 D16S539: 12 D5S818: 12,13 D7S820: 10, 13 THO1: 7, 9.3 TPOX: 6, 11 vWA: 17, 18 |
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| Comments: | This line was derived from BEAS-2B cells (see ATCC CRL-9609 ) by infection with the recombinant retrovirus Zip-neo-v-Ha-ras (constructed by recombining the pZipNeoSV(X) with a fragment containing the v-Ha-ras oncogene. Cells were selected in medium containing G418. The line is reported to be highly tumoriogenic, and to stain positively for keratins and SV40 T antigen. The cells can be used to screen chemical and biological agents for activity as growth factor, carcinogens, mutagens, etc. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line (BEBM) along with all the additives can be obtained from Lonza/Clonetics Corporation as a kit: BEGM, Kit Catalog No. CC-3170. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with the BEGM kit. Note: Do not filter complete medium. Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, add 0.25%trypsin-0.53mM EDTA with 0.5% PVP (polyvinylpirrolidone) solution and allow the culture to sit at room temperature until the cells begin to detach (usually 5 to 10 minutes). Add fresh medium, wash by centrifugation, aspirate and dispense into new flasks. Inoculate new flask at 1500 to 3000 cells per sq. cm. The flasks used should be precoated with with a mixture of 0.01 mg/ml fibronectin, 0.03 mg/ml bovine collagen type I and 0.01 mg/ml bovine serum albumin (see above reference). The cells should be subcultured before reaching confluence since confluent cultures rapidly undergo squamous terminal differentiation. Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Leibovitz?s L-15 medium with 2 mM L-glutamine supplemented with 10 mM HEPES, 1% PVP, 10% fetal bovine serum and 7.5% DMSO Storage temperature: liquid nitrogen vapor phase |
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| References: | 21937: Reddel RR, et al. Immortalized human bronchial epitherial mesothelial cell lines. US Patent 4,885,238 dated Dec 5 1989 22301: Lechner JF, LaVeck MA. A serum-free method for culturing normal human bronchial epithelial cells at clonal density. J. Tissue Culture Methods 9: 43-48, 1985. |
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