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- 细胞形态：
原始粒细胞
- 相关疾病：
其他疾病
- ATCC Number：
CCL-246.1™
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 库存：
大量
- 运输方式：
冻存运输
- 年限：
59 years
- 生长状态：
悬浮生长
- 器官来源：
骨髓
| Designations: | KG-1a | ||
| Depositors: | DW Golde | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | myeloblast |
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| Source: | Organ: bone marrow Disease: acute myelogenous leukemia |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Antigen Expression: | HLA A30, A31, B35, Cw4 | ||
| DNA Profile (STR): | Amelogenin: X,Y CSF1PO: 7 D13S317: 11,12 D16S539: 10,11 D5S818: 13 D7S820: 8,10 THO1: 7,8 TPOX: 7,9 vWA: 14,19 |
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| Cytogenetic Analysis: | The stemline chromosome number is 46 (pseudodiploid), with the 2S component occurring at 5.8%. Seven markers, including five ATCC CCL-246-specific markers, were found in most, if not all metaphases analyzed., Another marker ? del (7) was found only in about 50% of the metaphases. Normal chromosomes 5, 7, 8, 12, 17 and 22 were monosomic. The Y chromosome is detected in the Q-banded preparations. | ||
| Isoenzymes: | AK-1, 0 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1-2 PGM3, 0 |
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| Age: | 59 years | ||
| Gender: | male | ||
| Ethnicity: | Caucasian | ||
| Comments: | The variant subline KG-1a of the human acute myelogenous leukemia cell line KG-1 was isolated by H.P. Koeffler, et al. After the tenth passage, the parental KG-1 cells were cultured in two separate laboratories within the same department under identical conditions. After 35 passages the cells in one laboratory expressed morphological differences from the parent line. The variant KG-1a was composed of undifferentiated promyeloblasts. The cells did not stain for ASD chloroacetate esterase, alpha-naphthyl butyrate esterase or peroxidase. Both populations exhibit many common characteristics. They share a similar doubling time, are negative for EBNA and VCA, express no surface immunoglobulins and exhibit identical HLA and isoenzyme profiles. In contrast to the parental KG-1 (ATCC CCL-246 ) the KG-1a population is unresponsive to colony-stimulating factor in soft-agar culture and does not express the Ia-like antigen. KG-1a cells are resistant to phorbol diester induced macrophage differentiation and proliferation of the cells is unaffected by the presence of phorbol diesters. The KG-1a cells are morphologically, cytochemically, and functionally less mature than the parental KG-1. Although derived from, and almost identical to, KG-1 (ATCC CCL-246 ) these cells do not spontaneously differentiate to granulocyte and macrophage like cells, do not express DR and do not respond to colony stimulating factor (CSF). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Temperature: 37.0°C |
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| Subculturing: | Medium Renewal: Twice per week Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005 recommended serum:ATCC 30-2020 |
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| References: | 1055: Koeffler HP. Induction of differentiation of human acute myelogenous leukemia cells: therapeutic implications. Blood 62: 709-721, 1983. PubMed: 6192859 22611: Koeffler HP, et al. An undifferentiated variant derived from the human acute myelogenous leukemia cell line (KG-1). Blood 56: 265-273, 1980. PubMed: 6967340 32395: Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679 33167: Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022 |
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: 1 M Tris pH 8.8 (do not use pH meter, store at room temp.) 1.23 g Tris HCl 5.13 g Tris base q.s. 50 mL with H2 O KG-1 (10x) (Store frozen) Amt [final] 8.3 mL 1M (NH
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KG-1a
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