![SW 626 [SW-626, SW626]](https://custom.dxycdn.com/trademd/upload/pic/2012/03/29/1332499969.jpg!small.t.1)
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- 详细信息
- 技术资料
- 相关疾病：
腺癌
- ATCC Number：
HTB-78™
- 细胞形态：
上皮样
- 年限：
grade III
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 器官来源：
卵巢
- 库存：
大量
- 生长状态：
贴壁生长
- 运输方式：
冻存运输
| Designations: | SW 626 [SW-626, SW626] | ||
| Depositors: | A Leibovitz | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: ovary Tumor Stage: grade III Disease: adenocarcinoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: January, 1974 | ||
| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 10,12 THO1: 9.3 TPOX: 8 vWA: 17,18 |
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| Cytogenetic Analysis: | Hypertetraploid; modal number = 104. The rate of higher ploidies was 23%. The markers der(2)t(2;5)(q35;q31); del(8)(q13q22); del(12)(q13); t(q9q13) and two others were common to most cells. Generally there were two copies of der(2) and three copies of del(8) per cell. The t(3;11)(p21;q25) and i(15q) markers were seen in some cells. Many cells had 8 copies of N3, N7, N9, N19 and N20, but only two copies of N2. Normal 8 was absent. There were four copies of X, and Y was not found. | ||
| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1 PGM1, 1 PGM3, 1 |
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| Age: | 46 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | Although originally thought to be of ovarian origin, a recent report has suggested that the SW 686 cell line might have been derived from an ovarian metastasis of a primary adenocarcinoma of the colon. The SW 626 cell line was initiated by A. Leibovitz in January 1974 at the Scott and White Clinic, Temple, Texas from a surgical specimen from a cystadenocarcinoma of the ovary in a 46 year old female Caucasian. The histopathology of the specimen was determined to be grade III adenocarcinoma. A frozen ampule at passage 10 was received at the ATCC in January, 1982. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Atmosphere: air, 100% Temperature: 37.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008 recommended serum:ATCC 30-2020 |
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| References: | 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 32824: Strobel T, et al. BAX enhances paclitaxel-induced apoptosis through a p53-independent pathway. Proc. Natl. Acad. Sci. USA 93: 14094-14099, 1996. PubMed: 8943066 40013: Furlong MT, et al. Evidence for the colonic origin of ovarian cancer cell line SW626. J. Natl. Cancer Inst. 91: 1327-1328, 1999. PubMed: 10433623 |
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SW 626 [SW-626, SW626]
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