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- 相关疾病：
正常
- ATCC Number：
CRL-2285™
- 运输方式：
冻存运输
- 物种来源：
狗
- 是否是肿瘤细胞：
0
- 细胞形态：
上皮样
- 生长状态：
贴壁生长
- 库存：
大量
- 年限：
adult
- 器官来源：
肾脏
| Designations: | Super Tube | ||
| Depositors: | RJ Klebe | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Canis familiaris | ||
| Morphology: | epithelial |
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| Source: | Organ: kidney Disease: normal |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Age: | adult | ||
| Gender: | female | ||
| Comments: | Super Tube is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34 ) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel. Super tube forms large numbers of tubules when maintained at high cell density (cells are reported to form tubules within 3 days when plated at 7 x 10exp5 cells/well of a 24 well plate). For tubule formation, the cells should be fed twice a day by a complete medium change. Super Tube has a much lower resting level of cyclic-AMP and responds to forskolin stimulation of adenylate cyclase less readily than the original cell line; it also has a lower transepithelal resistance than both Super Dome (ATCC CRL- 2286) and MDCK. |
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| Propagation: | ATCC complete growth medium: These cells are grown in a medium containing a 1:1 mixture of Dulbecco's Modified Eagle's Medium and Ham's F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES, 0.5 mM sodium pyruvate, and 1.2 g/L sodium bicarbonate supplemented with 0.05 mM non-essential amino acids and 10% fetal bovine serum. Temperature: 37.0°C |
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| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 recommended serum:ATCC 30-2020 parental cell line:ATCC CCL-34 derived from same cell line:ATCC CRL-2286 |
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| References: | 38915: Klebe RJ, et al. Cyclic-AMP deficient MDCK cells form tubules. J. Cell. Biochem. 59: 453-462, 1995. PubMed: 8749715 | ||
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花粉萌发形成的管状结构。萌发时，花粉先吸收水分而膨胀，原生质开始流动，花粉的内壁从萌发孔露出，作管状延伸。大多数的球果植物，可因极度膨胀的外壁裂开而脱离，但松属（ Pinus）则只裂开不脱离。管内原生质流动显著，核也进入管中。原生质和核向管端移动，形成胼胝质栓（ calloseplug），与老的部分隔断，原生质流动只在胼胝质栓前面的管内进行。一般花粉管是一条，但也有伸出几条的，蜀葵（ Althaea rosea）约生 10条。也有分枝的 [松树
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