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- 详细信息
- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 细胞形态：
其他
- ATCC Number：
CRL-1821™
- 生长状态：
贴壁生长
- 器官来源：
胚胎
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 品系：
129/Ola
- 细胞类型：
其他细胞类型
- 年限：
embryo, blastocyst embryo, blastocyst
- 库存：
大量
| Designations: | ES-E14TG2a | ||
| Depositors: | T Doetschman | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | spherical |
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| Source: | Organ: embryo Cell Type: embryonic stem cell , blastocyst derived Strain: 129/Ola |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Age: | embryo, blastocyst embryo, blastocyst | ||
| Comments: | This is a derivative of one of several embryonal stem cell (ES) lines developed by M. Hooper in 1987. The cells are deficient in HGPRT (HPRT) and are resistant to 0.06 mM 6-thioguanine. The cells remain undifferentiated when cultured on feeder layers (embryonic fibroblasts or STO cells). The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer. When injected into blastocysts, the cells can colonize the germline. After conventional molecular gene modification techniques, they may be used to reconstitute mouse embryos. They may also be of use in studies of early post implantation structures. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.1 mM ; fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution. Allow the trypsin to loosen the cells, add fresh medium, aspirate and dispense into new flasks containing pre-plated irradiated feeder cells. Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 feeder layer cells:ATCC 56-X |
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| References: | 22254: Smith AG, Hooper ML. Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells. Dev. Biol. 121: 1-9, 1987. PubMed: 3569655 22923: Kuehn MR, et al. A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice. Nature 326: 295-298, 1987. PubMed: 3029599 22925: Doetschman T, et al. Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 330: 576-578, 1987. PubMed: 3683574 |
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文献和实验Growing feeder-independent embryonic stem cells§
(CGR8 and E14Tg2A) were established from delayed blastocysts on gelatinized tissue culture dishes in ES cell medium containing leukocyte inhibitory factor (LIF) (Nichols et al., 1990). Sublines were isolated by plating cells at a single-cell density
Generation of a Series of Knock-In Alleles Using RMCE in ES Cells
. Here, an experimental procedure for the application of RMCE in E14 ES cells using heterotypic FRT recombination target sequences and the site-specific recombinase Flp is elaborated.
胚胎干细胞的转染而设计的。胚胎干细胞的研究持续升温，但转染始终是个令人头疼的问题。胚胎干细胞似乎“百毒不侵”，抗拒着外来的DNA。Xfect mESC则是从2300多个候选者中脱颖而出的佼佼者，在干细胞转染上有着不俗的表现。经过验证，它在小鼠胚胎干细胞系E14TG2A 和D3中的转染效率均高于市场上畅销的其他试剂。Xfect mESC的价格为2375元/100次。 之后，Clontech又为人成体干细胞研究推出了Xfect™ Adult Stem Cell转染试剂。它的配方经过修改，能在人间
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