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- 细胞形态：
上皮样
- ATCC Number：
CRL-1583™
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
- 器官来源：
胎盘
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
SV40转化细胞
- 库存：
大量
| Designations: | 3A(tPA-30-1) | ||
| Depositors: | J Chou | ||
| Biosafety Level: | 2 [Cells contain polyomavirus DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: placenta Cell Type: SV40 transformed |
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| Cellular Products: | at 40C the cells synthesize human chorionic gonadotropin (hCG) and alkaline phosphatase | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 9,12 D5S818: 11,13 D7S820: 10 THO1: 9,9.3 TPOX: 8,11 vWA: 16,17 |
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| Cytogenetic Analysis: | This is a hypodiploid human cell line with the modal chromosome number of 42 occurring in 34% of cells. However, cells with 44 chromosomes also occurred at a high frequency (26%). Most cells contained 41-45 chromosomes. Cells having a higher ploidy occurred at 20.7%, which is very high. Most cells averaged five or six marker chromosomes, none of which appeared twice in the cells examined. No chromosomes were paired consistently. | ||
| Comments: | The cells are transformed by SV40 ts30. The cells express the transformed phenotype at the permissive temperature (33C) and the non-transformed phenotype at the non-permissive temperature (40C). The line has a limited life expectancy of 15 to 18 passages before entering the crisis stage. |
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| Propagation: | ATCC complete growth medium: Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10% Temperature: 33.0°C |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | recommended serum:ATCC 30-2020 | ||
| References: | 1102: Chou JY. Human placental cells transformed by tsA mutants of simian virus 40: a model system for the study of placental functions. Proc. Natl. Acad. Sci. USA 75: 1409-1413, 1978. PubMed: 206898 | ||
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文献和实验Adding 3A overhang to a PCR product
Procedure Purify the PCR product. Before adding the overhangs it is very important to remove all the Proofreading DNA Polymerase (Pfu) by purifying the PCR product carefully (e.g. with a commercial PCR purification kit or phenol
小鼠 补体3a（C3a） 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定小鼠血清，血浆及相关液体样本中 补体3a(C3a) 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 小鼠 补体3a （ C3a ） 水平。用纯化的 小鼠 C3a 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 补体3a （ C3a ）， 再与HRP 标记的 C3a 抗体
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠 Sema3A(Sema3A)ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 Sema3A 单抗包被于酶标板上，标准品和样品中的 Sema3A 与单抗结合，加入生物素化的抗大鼠 Sema3A ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合
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