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- 2026年06月21日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 器官来源：
胎盘
- 生长状态：
贴壁生长
- 库存：
大量
- ATCC Number：
CRL-1584™
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 细胞类型：
SV40转化细胞
| Designations: | 3A-sub E [post crisis of 3A(tPA-30-1)] | ||
| Depositors: | MI Cour | ||
| Biosafety Level: | 2 [Cells contain polyomavirus DNA sequences ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens | ||
| Morphology: | |
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| Source: | Organ: placenta Cell Type: SV40 transformed |
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| Cellular Products: | at 40C the cells produce human chorionic gonadotropin (hCG) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 9,12 D5S818: 11,13 D7S820: 10 THO1: 9,9.3 TPOX: 8,11 vWA: 16,17 |
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| Comments: | This line was established from ATCC CRL-1583 cells that had begun to senesce. By passage 20 the cells had recovered and appeared capable of unlimited proliferation. The saturation density (6 X 10 exp5 cells/sq cm) is increased and the morphology is altered relative to the parental line. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended Medium Renewal: 1 to 2 times per week Remove medium, rinse culture with fresh 0.25% trypsin-0.03% EDTA solution and let sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
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| Preservation: | culture medium 95%; DMSO, 5% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 |
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文献和实验Expression of Recombinant Cytochromes c in E. coli
electron transfer proteins carrying one or more hemes covalently bound to the polypeptide chain – are one of the most recalcitrant classes of proteins with respect to heterologous expression because post-translational incorporation of hemes is required
and associatedactivity. This protocol describes the purification of Wnt3A specifically;modifications for other Wnt proteins are necessary (e.g.,elution profiles may vary; for detection use the appropriate Wntantibody; not all Wnts stabilize b-catenin
that disrupt GSK-3 signaling (e.g., genetargetedcells such as mouse embryonic fibroblasts [MEFs] lacking GSK-3a or GSK-3b, or RNA interference techniques) when examining the involvement of GSK-3 isoforms in the regulationof specific cellular processes
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