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- 2026年06月19日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
其他
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 年限：
2 years old
- 库存：
大量
- 细胞形态：
其他
- ATCC Number：
CRL-2996™
- 器官来源：
外周血
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
| Designations: | EML-3C | ||
| Depositors: | R Montelaro | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Equus caballus | ||
| Morphology: | macrophage-like |
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| Source: | Organ: peripheral blood Cell Type: macrophage-like Breed: Portuguese autochthonous (Garrano) |
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| Cellular Products: | non-specific esterase produces nitrites in response to LPS stimulation |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: May 2005 limiting dilution |
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| Applications: | study of interaction between EIAV and macrophages; lentiviruses persistence; cytopathicity mechanisms | ||
| Receptors: | equine infectious anemia virus (EIAV) cellular receptor, expressed | ||
| Virus Susceptibility: | Equine infectious anemia virus | ||
| Antigen Expression: | CZ2.2+, Ki-M6 (CD68)+, MHC-I+, MHC-II+ | ||
| Age: | 2 years old | ||
| Gender: | male | ||
| Comments: | The EML-3C cell line was established in 2005 from isolated peripheral blood mononuclear cells of a two-year-old male Portuguese Garrano horse. These cells possess functional properties of macrophages such as non-specific esterase (NSE) activity, are able to phagocytose fluorescent bioparticles and produce nitrites in response to lipopolysaccharide (LPS) stimulation. The EML-3C cell line expresses the EIA V receptor for cellular entry (ELR1) and supports replication of the virulent equine infectious anemia virus (EIA Vpv ). This cell line can be used as a valuable tool for studying equine macrophage functions, lentivirus infection, and the equine immune system. [16173840 ] | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to 500 ml of the base medium:
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| Subculturing: | Protocol: Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
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| Preservation: | Freeze medium: complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2002 Recommended fetal bovine serum: ATCC 30-2020 Recommended horse serum: ATCC 30-2040 Trypsin EDTA Solution: ATCC 30-2101 Phosphate-buffered saline: ATCC 30-2200 Cell culture tested DMSO: ATCC 4-X MEM Non-Essential Amino Acid Solution, 100x, ATCC 30-2116 |
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| References: | 16173840: Isabel Fidalgo-Carvalho et al. Characterization of an equine macrophage cell line: application to studies of EIAV infection. Vetenary Microbiol. 136 (1-2): 8?19, 2009. PubMed: 19038510 | ||
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文献和实验相关实验
广义的细胞色素 c是含有血红素 c的细胞色素之总称。通常是指主要存在于高等动植物、酵母、霉菌等线粒体中的细胞色素 c，起着传递电子的作用。为分子量约 1.3万的碱性蛋白质，还原型的吸收带为 550、 520、 415毫微米，氧化型为 407毫微米。α 因此很早便被研究，并获得结晶。不仅一级结构而且立体结构也已清楚（图 1）。其结构基本上是生物界共有的。由细胞色素 c氧化酶〔细胞色素（ a＋ a3 ）复合体〕氧化。在呼吸链中从细胞色素 c开始接受电子，各种还原酶都已清楚
也称为底土层，一般不受成土因子的影响，因而是一般不具有 A层和 B层特性的土壤层次，在 A层或 B层发达的土壤中，在这些层次之下存在有 C层。
主要显示着丝粒结构异染色质, 及其它区段的异染色质部分。标本可用酸 (HCl)及碱〔Ba(OH)2 〕变性处理, 再经2xSSC在60℃中温育1小时, 最后用Giemsa染料染色显带。
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EML-3C
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