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- 技术资料
- 生长状态：
贴壁生长
- 器官来源：
外周血
- 相关疾病：
其他疾病
- ATCC Number：
CRL-2256™
- 品系：
Wistar
- 运输方式：
冻存运输
- 细胞形态：
成纤维样
- 库存：
大量
- 物种来源：
大鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
| Designations: | RBL-2H3 | ||
| Depositors: | RP Siraganian | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Rattus norvegicus deposited as Rattus sp. | ||
| Morphology: | fibroblast |
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| Source: | Strain: Wistar Organ: peripheral blood Disease: basophilic leukemia Cell Type: basophil; chemically induced |
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| Cellular Products: | histamine | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Receptors: | FcERI (Fc of IgE) | ||
| Comments: | RBL-2H3 is a basophilic leukemia cell line isolated and cloned in 1978 in the Laboratory of Immunology at the National Institute of Dental Research from Wistar rat basophilic cells that were maintained as tumors. These cells have high affinity IgE receptors. They can be activated to secrete histamine and other mediators by aggregation of these receptors or with calcium ionophores. They have been used extensively to study FcERI and the biochemical pathways for secretion in mast cells. RBL-2H3 cells have been the model for studies of structure of FcERI. They have been used extensively for studies of different aspects of secretion in cells including the role of changes in intracellular calcium, the activation of phospholipases, protein kinases and small G proteins. Although nearly all lots of fetal bovine serum support the growth of these cells, the cells grown in some lots degranulate better after FcERI aggregation. Another rat basophil line is available (RBL-1, see ATCC CRL-1378 ) that does not degranulate. Histamine release capacity may be seriously reduced after too much subculturing. PubMed: 6166481. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol:
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 | ||
| References: | 1232: Kulczycki A Jr., et al. The interaction of IgE with rat basophilic leukemia cells. I. Evidence for specific binding of IgE. J. Exp. Med. 139: 600-616, 1974. PubMed: 4812630 22475: Barsumian EL, et al. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur. J. Immunol. 11: 317-323, 1981. PubMed: 6166481 22638: Eccleston E, et al. Basophilic leukaemia in the albino rat and a demonstration of the basopoietin. Nat. New Biol. 244: 73-76, 1973. |
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文献和实验相关实验
Bruton's tyrosine kinase (Btk) (33) NM_000061 1682-1700 AAUUGGUAAACGAUCAAGGAG RBL-2H3, WEHI-231, NALM
, hematopoetic cells beta-tubulin- mouse neuronal (21) AF312873 AAGACAGAGCCAAGUGGACUC Mouse P19 Bruton's tyrosine kinase (Btk) (33) NM_000061 1682-1700 AAUUGGUAAACGAUCAAGGAG RBL-2H3, WEHI-231, NALM-6 Bruton's tyrosine kinase (Btk) (33) NM_000061 895-913
后 6 周仍然维持较高水平重建。 图 5：植入人外周血中 NK 细胞后 B-NDG hIL-15 小鼠中重建 NK 细胞的数量和比例[5] B-NDG 小鼠在 PDX 中的应用 2022 年 Cancer Letters 中使用 B-NDG 小鼠胰腺癌 PDX 模型，证明了 HOXB9 通过上调 RBL2 和抑制 c-Myc 阻断 G0/G1 期的细胞周期进程，DNMT1 使得 HOXB9 甲基化并下调其表达。 图 6：Cancer Lett.2022;533
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RBL-2H3
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