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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 年限：
60 years
- 运输方式：
冻存运输
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 组织来源：
right frontal parieto-occipital cortex
- 相关疾病：
其他疾病
- ATCC Number：
CRL-2611™
- 生长状态：
贴壁生长
- 器官来源：
大脑
- 细胞形态：
上皮样
- 库存：
大量
| Designations: | LN-229 | ||
| Depositors: | N de Tribolet | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Homo sapiens deposited as human | ||
| Morphology: | epithelial |
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| Source: | Organ: brain Tissue: right frontal parieto-occipital cortex Disease: glioblastoma |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1979 | ||
| Tumorigenic: | Yes | ||
| Oncogene: | p53 + (mutated, CCT (Pro) --> CTT (Leu) mutation at codon 98), PTEN + (wild type), p16 - (deleted), p14ARF - (deleted) | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 12 D13S317: 10,11 D16S539: 12 D5S818: 11,12 D7S820: 8,11 THO1: 9.3 TPOX: 8 vWA: 16,19 |
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| Age: | 60 years | ||
| Gender: | female | ||
| Ethnicity: | White | ||
| Comments: | The cells exhibit mutated p53 (TP53) and possible homozygous deletions in the p16 and p14ARF tumor suppressor genes. They have a wild-type PTEN gene. [51573 ] The LN-229 cell line was established in 1979 from cells taken from a patient with right frontal parieto-occipital glioblastoma [PubMed. 10416987]. [51573 ] Stimulation of the cells with Fas ligand lead to apoptotic cell death within 16 hours. The cells were also killed by puromycin in a dose dependent manner. [53380 ] Bcl-2 protects these cells from Fas ligand-induced cell death but was shown to have only a small protective effect on puromycin-induced apoptosis. [53380 ] This cell line is used in studies on apoptosis. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%. Temperature: 37.0°C |
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| Subculturing: | Protocol:
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| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 51571: Diserens AC, et al. Characterization of an established human malignant glioma cell line: LN-18. Acta Neuropathol. 53: 21-28, 1981. PubMed: 7211194 51573: Ishii N, et al. Frequent co-alterations of TP53, p16/CDKN2A, p14ARF, PTEN tumor suppressor genes in human glioma cell lines. Brain Pathol. 9: 469-479, 1999. PubMed: 10416987 53380: Schlapbach R, Fontana A. Differential activity of bcl-2 and ICE enzyme family protease inhibitors on Fas and puromycin-induced apoptosis of glioma cells.. Biochim. Biophys. Acta 1359: 174-180, 1997. PubMed: 9409814 |
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文献和实验表面受体结合的结构域。正是这些独立的结合位点使LN作为一个桥梁分子，介导细胞同基膜结合。所以LN的主要功能就是作为基膜的主要结构成分对基膜的组装起关键作用, 在细胞表面形成网络结构并将细胞固定在基膜上。 LN还有许多其他的作用,如在细胞发育过程中刺激细胞粘着、细胞运动。LN还能够刺激胚胎中神经轴的生长,并促进成年动物的神经损伤后重生长和再生。如同纤粘连蛋白,细胞外的LN能够影响细胞的生长、迁移和分化。LN在原生殖细胞的迁移中起关键作用。
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Titration of Human Coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay
229E and OC43 of human coronavirus (HCoV). An alternative indirect immunoperoxidase assay (IPA) is herein described for the detection and titration of these viruses. Susceptible cells are inoculated with serial logarithmic dilutions of samples in a 96
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