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- 详细信息
- 技术资料
- 库存：
大量
- ATCC Number：
SCRC-1040™
- 品系：
CF-1
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
成纤维细胞
- 器官来源：
胚胎
- 年限：
14 days gestation embryo
- 细胞形态：
成纤维样
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
| Designations: | MEF (CF-1) | ||
| Depositors: | ATCC | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus | ||
| Morphology: | fibroblast |
||
| Source: | Organ: embryo Strain: CF-1 Cell Type: fibroblast |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 2003 | ||
| Applications: | The cells can be used as a feeder layer to support the growth of embryonic stem (ES) cells and for the maintenance of ES cells in the undifferentiated state. ATCC has successfully irradiated (SCRC-1040.1) and treated the cells with Mitomycin C (SCRC-1040.2a) for use as a feeder layer. The cell line was established by ATCC in 2003 from embryonic day 14 (E14) CF-1 mouse embryos. The growth of these cells should be arrested before being used as a feeder layer. If the MEFs are being used as a feeder layer for ES cells, it is not recommended to use them past passage no.6 (P6) |
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| Age: | 14 days gestation embryo | ||
| Gender: | male and female mixed | ||
| Comments: | The cell line was established by ATCC in 2003 from embryonic day 14 (E14) CF-1 mouse embryos. The cells can be used as a feeder layer to support the growth of embryonic stem (ES) cells and for the maintenance of ES cells in the undifferentiated state. The growth of these cells should be arrested before being used as a feeder layer. ATCC has successfully irradiated (SCRC-1040.1) and treated the cells with Mitomycin C (SCRC-1040.2a) for use as a feeder layer. If the MEFs are being used as a feeder layer for ES cells, it is not recommended to use them past passage no. 6 (P6). | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
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| Subculturing: | Protocol: Establishing and maintaining your culture: To insure the highest level of viability, be sure to warm media to 37�C before using it on the cells. Flasks do not need to be coated before plating MEFs.
To insure the highest level of viability, be sure to warm media and Trypsin / EDTA to 37�C before using it on the cells. Cells should be split when they reach confluency. Split cells at approximately 0.4 X 104 cells/cm2
Subcultivation Ratio: Plate the cells at approximately of 0.4 X 104 cells/cm2 . Medium Renewal: Twice a week or when pH decreases. |
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| Preservation: | Freeze medium: Complete growth medium supplemented with an additional 40% FBS and 10% DMSO Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 derivative:ATCC SCRC-1040.1 derivative:ATCC SCRC-1040.2a |
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| References: | 89421: Nagy A, et al. Manipulating The Mouse Embryo: A Laboratory Manual. Third Edition: Cold Spring Harbor Press; 2003. | ||
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MEF (CF-1)
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