- 询价
- 2025年11月02日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 库存：
大量
- 细胞形态：
淋巴样
- ATCC Number：
CRL-1992™
- 运输方式：
冻存运输
- 生长状态：
悬浮生长
| Designations: | T2 (174 x CEM.T2) | ||
| Depositors: | P Cresswell | ||
| Biosafety Level: | 2 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Homo sapiens | ||
| Morphology: | lymphoblast |
||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host (Roche Transfection Reagents ) | ||
| Antigen Expression: | HLA A2; CD7 + | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 11, 12 D13S317: 11, 12 D16S539: 10, 12 D5S818: 12, 14 D7S820: 10, 11, 15 THO1: 6, 7, 9.3 TPOX: 8, 12 vWA: 14, 17, 20 |
||
| Comments: | This line is a variant of the T1 cell line (ATCC CRL-1991 ) produced by selection the SFR1-MI.3 monoclonal antibody (against a monomorphic determinant on HLA DR). The cells do not express HLA DR and are Class II major histocompatibility (MHC) antigen negative. The cells synthesize, but do not express, HLA B5. T2 appears to have lost both copies of the CEMR.3 derived chromosome 6. Together the T1 and T2 lines are useful for studying antigen processing and T cell recognition of class I MHC antigens. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Temperature: 37.0°C |
||
| Subculturing: | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) viable cells/ml and maintain between 3 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Every 2 to 3 days |
||
| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005 recommended serum:ATCC 30-2020 |
||
| References: | 22647: Salter RD, et al. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics 21: 235-246, 1985. PubMed: 3872841 | ||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验一光年 最近要做生殖的细胞方面的实验，前期一直用在垂体细胞原代培养，但是一直困扰没有促性腺激素细胞系，但是在网络中搜索，发现老外的文章中经常提到αT3-1，LβT2 或LβT4细胞系，在ATCC等知名的细胞库也未发现这些细胞系，不知道各位高手指点在哪里可以找到这几种细胞系？不甚感激！！ veimojie 我一直也在做这方面的研究 什么地方能有啊 ？ banana_smile 我也想做这方
The 5T2MM Murine Model of Multiple Myeloma: Maintenance and Analysis
of the myeloma cells into young syngeneic mice. We report methods involving the maintenance of the 5T2MM model and the quantification of tumor burden (by determining serum paraprotein concentration and plasmacytosis), assessment of bone lesions
1、打开电源：把电源开关板向上。 2、等待15～20分钟后打开测量开关：把测量开关板向上。 3、把“起辉-保持”开关向上扳至“直流”位置。 4、放入空白溶液。 5、调节调零旋钮使校荧屏上显示屏上显示为“0”。 6、倒掉空白液，放入试样液，即可在校影屏上读取数值。 7、分别按下左、右复测按钮一次，并读取读数，按几次，测量二平均值作为测量结果。 8、测量完毕，依次扳下“起辉-保持”、“测量”和“电源”按钮，拔下电源插头。
技术资料暂无技术资料 索取技术资料
