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- 运输方式：
冻存运输
- 库存：
大量
- 生长状态：
悬浮生长
- 免疫类型：
IgM
- 细胞形态：
淋巴样
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- ATCC Number：
HB-8710™
| Designations: | KC-4M1 [KC-4(IgM)] | ||
| Depositors: | Coulter Corporation | ||
| Isotype: | IgM | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human carcinoma cells | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with prostatic adenocarcinoma cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with an antigen present on solid tumors (98 of 104 tested) and cross-reacts with minimal cross reaction with paraffin embedded (14 of 92) or frozen (1 of 33) normal tissues. The antigen is found in the cytoplasm as a 490000 dalton molecule, and in the membrane and cytoplasm as a 438000 dalton molecule. An antibody with the same specificity but of the IgG3 subclass is available (ATCC HB-8709 ). |
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| Propagation: | ATCC complete growth medium: Modified Dulbecco's medium with 0.1 mM hypoxanthine and 0.015 mM thymidine; fetal bovine serum, 15% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | fetal bovine serum, 90%; DMSO, 10% | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X | ||
| References: | 3910: Kortright KH, Hofheinz DE. Monoclonal antibody to a human carcinoma tumor associated antigen. US Patent 4,708,930 dated Nov 24 1987 | ||
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠CXCL1/KC (KC)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液、唾液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 KC 单抗包被于酶标板上，标准品和样品中的 KC与单抗结合，加入生物素化的抗小鼠 KC
佚名 血清中IgM是由5个单体通过一个J链和二硫键连接成五聚体，分子量最大，为970kD，沉降系数为19S，称为巨球蛋白（macroglobulin）。在分子结构上IgM无铰链区，Cμ2可能替代了铰链区的功能。在生物进化过程中IgM是最早出现的免疫球蛋白，如八目鳗可产生IgM。在个体发育过程中，无论是B细胞膜表面Ig（SmIg），还是合成分泌到血清中的Ig
佚名 血清中IgM是由5个单体通过一个J链和二硫键连接成五聚体，分子量最大，为970kD，沉降系数为19S，称为巨球蛋白（macroglobulin）。在分子结构上IgM无铰链区，Cμ2可能替代了铰链区的功能。在生物进化过程中IgM是最早出现的免疫球蛋白，如八目鳗可产生IgM。在个体发育过程中，无论是B细胞膜表面Ig（SmIg），还是合成分泌到血清中的Ig
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