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- 2025年11月09日
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- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
人
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 细胞形态：
上皮样
- 年限：
70 years
- 器官来源：
乳房
- 库存：
大量
- ATCC Number：
HTB-123™
- 生长状态：
悬浮生长，多细胞聚集
| Designations: | DU4475 | ||
| Depositors: | AJ Langlois | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension, multicell aggregates | ||
| Organism: | Homo sapiens | ||
| Morphology: | epithelial |
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| Source: | Organ: mammary gland; breast | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | Numerous desmosomes and interdigitating microvilli were observed by transmission electron microscopy. This line grows in floating clusters and viability cannot be determined accurately with dye exclusion tests. |
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| Tumorigenic: | Yes | ||
| DNA Profile (STR): | Amelogenin: X CSF1PO: 9,12 D13S317: 11,14 D16S539: 11,12 D5S818: 11 D7S820: 9,10 THO1: 6,8 TPOX: 8 vWA: 17 |
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| Cytogenetic Analysis: | modal number = 93; range = 85 to 95. The cell line is aneuploid human female, with chromosome counts in the tetraploid range. Normal chromosomes N16 and N21 are least well represented, with from none to two copies per karyotype, while chromosome N7 tends to be over-represented, with four or five copies per karyotype, whereas two to four copies are found of most of the remaining normal chromosomes. Twenty-two marker chromosomes are found including: iso(1q), del(1)(q32), der(1)t(1;?)(p36;?), del(3)(q24), der(3)t(3;?)(q24;?), del(3)(p14p25), t(6q;21?) and others. Eight are identical to those reported or portrayed in the report by A.J. Langlois, et al. |
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| Isoenzymes: | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 1-2 PGM3, 1 |
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| Age: | 70 years | ||
| Gender: | female | ||
| Ethnicity: | Caucasian | ||
| Comments: | Numerous desmosomes and interdigitating microvilli were observed by transmission electron microscopy. This line grows in floating clusters and viability cannot be determined accurately with dye exclusion tests. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 2 X 10 exp5 cells/ml and maintain at between 10 exp5 and 10 exp6 cells/ml. Medium Renewal: 2 to 3 times per week |
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| Preservation: | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001 recommended serum:ATCC 30-2020 |
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| References: | 23034: Langlois AJ, et al. Morphological and biochemical properties of a new human breast cancer cell line. Cancer Res. 39: 2604-2613, 1979. PubMed: 221108 23093: Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216 |
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文献和实验指在同一土壤中连续种植同一种植物时，而不久植物就出现生长不良的现象，这种情况在比较有限的植物范围内常可见到。葫芦科、茄科、豆科等作物，适当轮作较之连作具有更好的效果。忌地的原因，考虑是多种多样：（ 1）出自植物根的生长障碍物质或植物残体分解的中间产物的形成，被残留于土壤中（豆科、葫芦科植物）：（ 2）病菌及害虫存在于土壤中，对后茬同种作物造成危害；（ 3）植物对需要程度不同的微量物质被吸收而感到不足时，对生长也有不良影响。
HGF-Induced DU145 Cell Scatter Assay
that is well known to induce such a conversion, termed “cell scattering”, in Madin Darby canine kidney (MDCK) cells. Recently, we have developed an alternative model of cell scattering using the human prostate cancer cell line, DU145. Like MDCK cells, DU145 cells
Sequencing Using the Du Pont Genesis 2000 DNA Analysis System
The Du Pont Genesis™ 2000 DNA Analysis System consists of an instrument, reagents capable of labeling DNA with novel fluorescent dideoxynucleotides (1 ), and a Macintosh® II computer to operate the instrument and perform all subsequent data
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