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- 询价记录
- 文献和实验
- 技术资料
- 运输方式：
冻存运输
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 生长状态：
悬浮生长，多细胞聚集
- 细胞类型：
其他细胞类型
- 免疫类型：
IgM , IgM (surface)
- 细胞形态：
淋巴样
- 相关疾病：
其他疾病
- ATCC Number：
CRL-1702™
- 库存：
大量
- 品系：
(BALB/c x NZB)F1
| Designations: | WEHI-231 | ||
| Depositors: | NL Warner, LL Lanier | ||
| Isotype: | IgM , IgM (surface) | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension, multicell aggregates | ||
| Organism: | Mus musculus | ||
| Morphology: | lymphoblast |
||
| Source: | Disease: B cell lymphoma Strain: (BALB/c x NZB)F1 Cell Type: B lymphocyte, immature; |
||
| Cellular Products: | immunoglobulin | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Comments: | WEHI-231 cells do not secrete IgM into medium unless stimulated with lipopolysaccharide (0.01 to 3 mcg/ml).Tested and found negative for ectromelia virus (mousepox). | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
||
| Subculturing: | Protocol: Optimum culture recovery from a frozen vial is in a T25 flask at a density between 2 to 5 X 10 (5) viable cells/ml. If recovered at 5 X 10 (5) viable cells/ml, media will need to be added to the culture within the first 3 days, before the density reaches 1 X 10 (6) viable cells/ml, at which point the viability drops drastically. After cells recover, expansions can be done at 1 X 10 (5) viable cells/ml by adding media to decrease the cell concentration. This cell line forms tight clusters of viable cells accompanied by some single non-viable cells plus debris in suspension. With increased growth the clusters become larger. As the cell density nears 1 X 10 (6) cells/ml, the single (non-clustered) and non-viable cells increase along with the amount of debris. At subculture, break up clusters by gentle pipetting. Established cultures can be grown in T75 flasks. Subcultivation Ratio: Add fresh medium every 2 to 3 days (depending on cell density) Medium Renewal: Every 2 to 3 days |
||
| Preservation: | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
||
| Doubling Time: | about 20 hours | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 Cell culture tested DMSO:ATCC 4-X |
||
| References: | 1224: Boyd AW, et al. The regulation of growth and differentiation of a murine B cell lymphoma. I. Lipopolysaccharide induced differentiation. J. Immunol. 126: 2461-2465, 1981. PubMed: 6785355 26147: Lanier LL, Warner NL. Cell cycle related heterogeneity of Ia antigen expression on a murine B lymphoma cell line:analysis by flow cytometry. J. Immunol. 126: 626-631, 1981. PubMed: 6969756 58054: Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803 |
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