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- ATCC Number：
HB-60™
- 免疫类型：
IgG2b; lambda light chain
- 细胞形态：
淋巴样
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 生长状态：
悬浮生长
- 运输方式：
冻存运输
- 库存：
大量
| Designations: | C3-124 | ||
| Depositors: | JF Kearney, H Kubagawa | ||
| Isotype: | IgG2b; lambda light chain | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human gamma heavy chain | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with human IgG. Spleen cells were fused with P3X63Ag8.653 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| References: | 931: Kuritani T, Cooper MD. Human B cell differentiation. I. Analysis of immunoglobulin heavy chain switching using monoclonal anti-immunoglobulin M, G, and A antibodies and pokeweed mitogen-induced plasma cell differentiation. J. Exp. Med. 155: 839-851, 1982. PubMed: 6801181 | ||
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文献和实验C 3 植物 C 3 plant 通过还原型戊糖磷酸循环而进行 CO 2 固定的植物称为 C 3 植物。例如：小球藻、菠菜、大豆、稻、小麦等都是 C 3 植物。已知在树木和藻类中只有 C 3 植物。除了 C 3 植物外，还有把 C4 二羧酸循环作为固定 CO 2 基本循环的 C 4 植物，以及通过还原型羧酸循环进行固定 CO 2 的某些光合细菌。 C3 植物在叶的微细构造、最大光合作用速度、有无瓦布效应（ Warburg ’ s ef － fect
补体成分 C3(C3a/C3b) 补体成分：C3 血清浓度(μg/ml)：550-1200 分子量(kDa)：195 亚单位(链)及分子量(kDa)：α∶110；β∶85 激活产物：C3a；C3b 生物学活性：C3a 过敏毒素、趋化作用等。 C3b 组成CP、AP中的C3、C5转化
网络 四、C3分子 C3处于两条激活途径的汇合点，在补体系统活化过程中起着枢纽作用，并为替代途径激活的关键分子。C3的α、β两条肽链组成，之间以二硫键相连结，分子量为195kDa，其中α链为115kDa，β链为75kDa（图5-6）。其在血清中的含量高于其它补体分子，约为0.55-1.2mg/ml。同C4分子一样，C3分子的α
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