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- 详细信息
- 技术资料
- ATCC Number：
HB-60™
- 免疫类型：
IgG2b; lambda light chain
- 细胞形态：
淋巴样
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 生长状态：
悬浮生长
- 运输方式：
冻存运输
- 库存：
大量
| Designations: | C3-124 | ||
| Depositors: | JF Kearney, H Kubagawa | ||
| Isotype: | IgG2b; lambda light chain | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
||
| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human gamma heavy chain | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with human IgG. Spleen cells were fused with P3X63Ag8.653 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| References: | 931: Kuritani T, Cooper MD. Human B cell differentiation. I. Analysis of immunoglobulin heavy chain switching using monoclonal anti-immunoglobulin M, G, and A antibodies and pokeweed mitogen-induced plasma cell differentiation. J. Exp. Med. 155: 839-851, 1982. PubMed: 6801181 | ||
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C3-124
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