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- 2026年07月29日
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- 详细信息
- 文献和实验
- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 运输方式：
冻存运输
- 生长状态：
悬浮生长
- ATCC Number：
HB-8759™
- 免疫类型：
IgG2a
- 细胞形态：
淋巴样
- 库存：
大量
| Designations: | XMMME-001 | ||
| Depositors: | Xoma Corporation | ||
| Isotype: | IgG2a | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against human melanoma tumor specific antigen | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | When cultures are seeded at 5 X 10(4) cells/ml, antibody production may not be detectable until days 6 or 7. Animals were immunized with M21 human melanoma cells. Spleen cells were fused with P3X63Ag8 myeloma cells. |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium: |
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| Subculturing: | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10(4) cells/ml and maintain between 1 X 10(5) and 2 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | recommended serum:ATCC 30-2020 Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X |
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| References: | 3561: Scannon PJ, et al. Human melanoma specific immunotoxins. US Patent 4,590,071 dated May 20 1986 22765: Levy JA, et al. Purification of monoclonal antibodies from mouse ascites eliminates contaminating infectious mouse type C viruses and nucleic acids. Clin. Exp. Immunol. 56: 114-120, 1984. PubMed: 6201307 |
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文献和实验-001” (FF2-6-001 qPCR set-up tool), and are in the process of transforming it into professional qPCR set-up software to be made available in 2007. The current prototype is already fully functional.
MHC I STREPTAMERS® 检测与分离抗原特异性 (CD8+ T) 细胞
Sequence Cat. No. Human Cytomegalovirus (CMV) HLA-A* 0201 CMV pp65 NLVPMVATV 6 - 7001 - 001 HLA-B* 0702 CMV pp65 TPRVTGGGAM
einstan001 最近本人正在做缺氧复氧诱导细胞凋亡实验，实验对象为贴壁细胞，诱导完凋亡后须收集细胞提RNA和蛋白并检测一些凋亡相关酶的活性与表达，在缺氧复氧后出现大量漂浮变圆的细胞，由于我采用采用胰酶消化的方法收集细胞后提RNA和蛋白，由于消化前需将原培基倾倒掉并用PBS洗涤细胞，因此这些漂起来变圆的细胞大量丢失，请问这些漂浮变圆的细胞我需要收集吗？这些细胞的丢失对我的实验结果影响大吗？谢谢 hggjoy 需要收集
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