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- 器官来源：
胸腺
- 相关疾病：
淋巴瘤
- ATCC Number：
TIB-48™
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 品系：
AKR/J
- 细胞类型：
T淋巴细胞
- 细胞形态：
淋巴样
- 运输方式：
冻存运输
- 库存：
大量
- 生长状态：
悬浮生长
| Designations: | BW5147.G.1.4 | ||
| Depositors: | R Hyman | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus | ||
| Morphology: | lymphoblast |
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| Source: | Organ: thymus Strain: AKR/J Disease: lymphoma Cell Type: T lymphocyte; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | This line was derived from BW5147.3 (ATCC TIB-47 ). The cells are resistant to 0.1 mM 6-thioguanine. Unlike the parental line, these cells are sensitive to HAT. Tested and found negative for ectromelia virus (mousepox). |
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| Antigen Expression: | H-2k | ||
| Comments: | This line was derived from BW5147.3 (ATCC TIB-47 ). The cells are resistant to 0.1 mM 6-thioguanine. Unlike the parental line, these cells are sensitive to HAT. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| References: | 1104: Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 22348: Kohler G, et al. Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reaction. Eur. J. Immunol. 7: 758-761, 1977. PubMed: 563329 22528: Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 26346: Goldsby RA, et al. Hybrid cell lines with T-cell characteristics. Nature 267: 707-708, 1977. PubMed: 301614 26347: Hammerling GJ. T lymphocyte tissue culture lines produced by cell hybridization. Eur. J. Immunol. 7: 743-746, 1977. PubMed: 304002 |
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文献和实验Generation of T Cell Hybridomas from Naturally Occurring FoxP3+ Regulatory T Cells
Generation of regulatory T cells (or Treg) derived hybridomas offers a tool to study their antigen specificity. T cells hybridomas are produced by fusing TCR α-β-thymoma BW5147 with highly dividing T cell population. In vitro anergy of Tregs
IM: 肌肉注射 IV: 静脉注射 SC: 皮下注射 IP: 腹腔注射药 物剂量途径麻醉前给药Atropine0.002-0.005 mg/100 g BW0.12 mg/100 g BWIM, IV, SC*IP*镇静剂AcepromazineDiazepamKetamine0.075 mg/100 g BW0.5 mg/100 g BW2.0 mg/100 g BWIMIPIM注射
0.002-0.005 mg/100 g BW 0.12 mg/100 g BW IM, IV, SC * IP * 镇静剂 Acepromazine Diazepam
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