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- 2026年05月06日
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- 文献和实验
- 技术资料
- 物种来源：
其他
- 是否是肿瘤细胞：
0
- 细胞形态：
成纤维样
- 细胞类型：
其他细胞类型
- ATCC Number：
CRL-9595™
- 运输方式：
冻存运输
- 库存：
大量
- 生长状态：
贴壁生长
| Designations: | MCB3901 [AV12-664] | ||
| Depositors: | Eli Lilly & Co. | ||
| Biosafety Level: | 2 [CELLS CONTAIN ADENOVIRUS ] | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mesocricetus auratus | ||
| Morphology: | fibroblast |
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| Source: | Cell Type: adenovirus 12 induced tumor | ||
| Cellular Products: | early region proteins of adenovirus 12 | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Cytogenetic Analysis: | modal chromosome number is approximately 46 | ||
| Comments: | This cell line can be used as a transfection host for exogenous gene expression using the BK virus enhancer - adenovirus late promoter system. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
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| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:8 to 1:20 is recommended Medium Renewal: Every 2 to 3 days Remove medium, rinse with fresh 0.25% trypsin, remove trypsin and incubate the flask at 37C until the cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. |
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| Doubling Time: | 14 to 16 hrs | ||
| References: | 21957: Beavers LS, et al. Recombinant and chimeric KS1/4 antibodies directed against a human adenocarcinoma antigen. US Patent 4,975,369 dated Dec 4 1990 23249: Berg DT, et al. E1a-responsive mammalian host/vector system for the stable high-level expression of secreted proteins. Nucleic Acids Res. 20: 5485-5486, 1992. PubMed: 1437573 23355: Grinnell BW, et al. Gamma-carboxylated isoforms of recombinant human protein S with different biologic properties. Blood 76: 2546-2554, 1990. PubMed: 2148275 23533: Burck PJ, et al. Characterization of a modified human tissue plasminogen activator comprising a kringle-2 and a protease domain. J. Biol. Chem. 265: 5170-5177, 1990. PubMed: 2108167 |
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文献和实验Characterization of Chromosomal Rearrangements Using Multicolor-Banding (MCB/m-band)
, more colorful, and thus, more informative. At present, the most frequently applied FISH banding technique is the multicolor banding (MCB/m-band) approach. MCB/m-band is based on region-specific microdissection libraries, producing changing fluorescence intensity
Overview of Adeno-Associated Viral Vectors
in a number of preclinical models of disease, including animal models of coagulopathies (11 ,12 ), lysosomal storage diseases (13 ,14 ), vision defects (15 ,16 ), and amino acid disorders (17 ). Clinical trials using A AV for the treatment of hemophilia
Cell Bank Preparation and Characterization
system. This consists of a master cell bank (MCB) of 50–400 ampoules and manufacturer’s working cell banks (WCB) each derived in an identical manner from one ampoule of the MCB (Table 1 ). In this chapter the methods of preparation, storage, and characterization
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