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- 文献和实验
- 技术资料
- 品系：
Buffalo
- 运输方式：
冻存运输
- 相关疾病：
其他疾病
- ATCC Number：
CRL-1601™
- 物种来源：
大鼠
- 是否是肿瘤细胞：
0
- 细胞形态：
上皮样
- 生长状态：
贴壁生长
- 库存：
大量
- 器官来源：
肝
| Designations: | McA-RH7777 | ||
| Depositors: | JE Becker | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | loosely adherent | ||
| Organism: | Rattus norvegicus deposited as Rattus sp. | ||
| Morphology: | epithelial |
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| Source: | Organ: liver Strain: Buffalo Disease: hepatoma; Morris hepatoma 7777 |
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| Cellular Products: | alpha-fetoprotein (AFP, alpha fetoprotein) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | transfection host | ||
| Receptors: | glucocorticoid | ||
| Gender: | female | ||
| Comments: | Addition of glucocorticoids (dexamethasone) to the medium accelerates cell proliferation and reduces alpha fetoprotein production. | ||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
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| Subculturing: | Protocol: Heavy monolayer sloughs off; subculture before 70% confluency. Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove culture medium with floating cells to a centrifuge tube.2. If any cells are attached, tap flask gently or if necessary add 2.0 to 3.0 ml of 0.25% Trypsin-0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed . 3. Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting. 4.To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximately 125 xg for 5 to10 minutes.5. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. 6. Place culture vessels in incubators at 37�C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 weekly is recommended Medium Renewal: Add medium every 2 to 3 days, do not discard floating cells. |
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| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
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| References: | 26103: . . Recent Results Cancer Res. 44: 103-114, 1974. 32449: Kulas DT, et al. The transmembrane protein-tyrosine phosphatase LAR modulates signaling by multiple receptor tyrosine kinases. J. Biol. Chem. 271: 748-754, 1996. PubMed: 8557682 32463: Schock D, et al. An auxiliary factor containing a 240-kDa protein complex is involved in apolipoprotein B RNA editing. Proc. Natl. Acad. Sci. USA 93: 1097-1102, 1996. PubMed: 8577721 58043: Becker JE, et al. Two new rat hepatoma cell lines for studying the unbalanced blocked ontogeny hypothesisIn: Becker JE, et al. Onco-developmental gene expression. New YorkAcademic Press, pp. 259-270, 1976 |
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Hyperplasia, RH）、改变复杂，其与恶性淋巴瘤在组织学方面鉴别很困难，误诊率可达10%～30%，是临床病理诊断中的难题。由于免疫细胞化学，分子生物学的发展，已有可能利用单克隆抗体（McAb）或多克隆抗体（PcAb）和IgH,TCR（T细胞受体）基因重排检测技术，将免疫活性细胞及其肿瘤细胞的细胞系及分化期、亚型、淋巴瘤良、恶性进行鉴别诊断。免疫细胞化学技术简单易行，具有一定的特异性。且可在组织原位标记。并可用于印片或细胞悬液，配合流式细胞仪，提供更为精细的信息。目前市场供应的McAb 与PcAb可已有百种
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McA-RH7777
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