- 询价
- 2026年09月08日
15 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
AJE 润色服务限时 8 折起，权威机构信任 15+ 年Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 细胞类型：
其他细胞类型
- 生长状态：
贴壁生长
- 年限：
newborn
- 细胞形态：
成纤维样
- ATCC Number：
CRL-2595™
- 库存：
大量
- 运输方式：
冻存运输
- 组织来源：
calvaria
- 器官来源：
骨
- 品系：
C57BL/6
| Designations: | MC3T3-E1 Subclone 24 | ||
| Depositors: | RT Franceschi | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Mus musculus deposited as mouse | ||
| Morphology: | fibroblast |
||
| Source: | Organ: bone Strain: C57BL/6 Tissue: calvaria Cell Type: preosteoblast; |
||
| Cellular Products: | collagen [51540 ] | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | These cell lines are good models for studying in vitro osteoblast differentiation, particularly ECM signaling. The MC3T3 Subclone 24 (ATCC CRL-2595 ) and the MC3T3 Subclone 30 (ATCC CRL-2596 ) lines exhibit poor osteoblast differentiation after growth in ascorbic acid. |
||
| Tumorigenic: | Yes | ||
| Age: | newborn | ||
| Comments: | A series of subclones were isolated from the cloned but phenotypically heterogeneous MC3T3-E1 cell line. The subclones were selected for high or low osteoblast differentiation and mineralization after growth in medium containing ascorbic acid. The MC3T3-E1 Subclone 4 (ATCC CRL-2593 ) and the MC3T3 Subclone 14 (ATCC CRL-2594 ) lines exhibit high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate. [51540 ] They form a well mineralized extracellular matrix (ECM) after 10 days. The MC3T3 Subclone 24 (ATCC CRL-2595 ) and the MC3T3 Subclone 30 (ATCC CRL-2596 ) lines exhibit poor osteoblast differentiation after growth in ascorbic acid. They do not form ECM. They can be used as negative controls for Subclones 4 and 14. [51540 ] Mineralizing subclones selectively express mRNAs for the osteoblast markers, bone sialoprotein (BSP), osteocalcin (OCN), and the parathyroid hormone (PTH)/parathyroid hormone-related protein (PTHrP) receptor. [51540 ] Subclones with both high and low differentiation potential produce similar amounts of collagen in culture and express comparable basal levels of mRNA encoding Osf2/Cbfa1, an osteoblast-related transcription factor. [51540 ] After implantation into immunodeficient mice, highly differentiating subclones form bone-like ossicles resembling woven bone, while poorly differentiating cells only produce fibrous tissue. [51540 ] These cell lines are good models for studying in vitro osteoblast differentiation, particularly ECM signaling. They have behavior similar to primary calvarial osteoblasts. |
||
| Propagation: | ATCC complete growth medium: The base medium for this cell line is Alpha Minimum Essential Medium with ribonucleosides, deoxyribonucleosides, 2 mM L-glutamine and 1 mM sodium pyruvate, but without ascorbic acid (GIBCO, Custom Product, Catalog No. A1049001). To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C |
||
| Subculturing: | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Every 2 to 3 days |
||
| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
||
| Related Products: | derived from same cell line:ATCC CRL-2593 derived from same cell line:ATCC CRL-2594 derived from same cell line:ATCC CRL-2596 |
||
| References: | 51540: Wang D, et al. Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential. J. Bone Miner. Res. 14: 893-903, 1999. PubMed: 10352097 | ||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
询价记录
暂无询价记录
文献和实验相关实验
(in water) and 25 μl of 24 mg/ml X-Gal (in DMF). 11. Add 2.5 ml of soft top agar to each sample, vortex and quickly pour onto the surface of a TYE-AMP agar plate. Allow at least 15-30 min. for the agar to solidify. 12. Invert the plates and incubate
of 24 mg/ml X-Gal (in DMF). 11. Add 2.5 ml of soft top agar to each sample, vortex and quickly pour onto the surface of a TYE-AMP agar plate. Allow at least 15-30 min. for the agar to solidify. 12. Invert the plates and incubate overnight at 37℃.
cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Passages: 17 Karyotype: Hypodiploid, modal No 20Hazard: CYA subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline
技术资料暂无技术资料 索取技术资料
MC3T3-E1 Subclone 24
询价
