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- 详细信息
- 技术资料
- 生长状态：
悬浮生长
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- ATCC Number：
HB-119™
- 运输方式：
冻存运输
- 免疫类型：
IgG1
- 细胞形态：
淋巴样
- 库存：
大量
| Designations: | ME 1 | ||
| Depositors: | P Parham | ||
| Isotype: | IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against HLA B7, Bw22, B27 | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with the JY human B lymphoblastoid cell line (HLA phenotype A11,A24,B7,B27) and with solubilized HLA A11, A24,B7,B27. Spleen cells were fused with NS-1 myeloma cells. The antibody cross-reacts with HLA B14 and HLA Bw46. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: Modified Dulbecco's medium with 0.1 mM hypoxanthine and 0.016 mM thymidine 90%; fetal bovine serum, 10% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | Freeze medium: complete growth medium, 95%; DMSO, 5%. | ||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X | ||
| References: | 978: Ellis SA, et al. Recognition of HLA-B27 and related antigen by a monoclonal antibody. Hum. Immunol. 5: 49-59, 1982. PubMed: 6981636 | ||
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ME 1
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