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- 细胞形态:
淋巴样
- 相关疾病:
多发性骨髓瘤
- ATCC Number:
TIB-17™
- 物种来源:
小鼠
- 是否是肿瘤细胞:
0
- 细胞类型:
B淋巴细胞
- 品系:
BALB/c
- 运输方式:
冻存运输
- 生长状态:
悬浮生长
- 库存:
大量
| Designations: | XS63 | ||
| Depositors: | D Schubert, K Horibata | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus | ||
| Morphology: | lymphoblast |
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| Source: | Disease: plasmacytoma; myeloma Strain: BALB/c Cell Type: B lymphocyte; |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Antigen Expression: | H-2d | ||
| Comments: | The XS63 cell line was established from the IgA secreting BALB/c myeloma. The cells do not secrete or synthesize immunoglobulin. Tested and found negative for ectromelia virus (mousepox). |
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| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| References: | 22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579 22522: Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881 26338: Schubert D, Horibata K. Immunoglobulin biosynthesis. II. Four independently isolated myeloma variants. J. Mol. Biol. 38: 263-271, 1968. PubMed: 4180612 26343: . . Cold Spring Harbor Symp. Quant. Biol. 32: 104, 1967. |
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文献和实验当师弟还在为有没有钱途,而纠结明年考不考博时,小编也不好替他拿主意。但看到一份文件后,小编立刻告诉他:你要读博!要去 G 省读!毕业后留在 G 省工作! 究竟是什么文件,让小编瞬间认为读博很有钱途? G 省又是哪个厚待人才的大省?广东人才计划原来是广东省出台的一份《关于加快新时代博士和博士后人才创新发展的若干意见》的通知,这份文件究竟有哪些吸引人的地方呢? 1. 每年补贴 200 名博士博后,每人 50 万,对,是生活补贴,可不是啥课题经费,有木有心动?小编是心动了。 2. 再看这一条
CD63 分子 CD63 常用单克隆抗体或代号: RUU- SP2.28,CLB 主要表达细胞: Pt,M,Mac,(G,T,B) [Pt] 分子质量(kDa)和结构: gp53,血小板颗粒蛋白(TM4- SF) 功 能: 血小板活化,中性粒细胞- 活化内皮细胞粘附 CD63 Aka lysosomal-membrane-associated glycoprotein 3, LAMP
摘 要一、人成骨肉瘤MG-63细胞株和成人成骨细胞的培养与分化特性的观察目的:观察人成骨肉瘤MG-63细胞株和正常成人成骨细胞分化的特性。方法:在细胞培养的不同时间,用α-磷酸奈酚法测定碱性磷酸酶(ALP)活性;放射免疫法测定骨钙素(BGP)含量;半定量逆转录聚合酶链反应(RT-PCR)测I型胶原、MMP-1、TIMP-1基因mRNA表达;Van GieSon氏苦味酸酸性复红染色法染色细胞I型胶原。结果:人成骨肉瘤MG-63细胞株接种培养第7天后I型胶原基因表达较高。MMP-1表达量随时间
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