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- 文献和实验
- 技术资料
- 库存：
大量
- 免疫类型：
IgG2b
- 细胞形态：
淋巴样
- ATCC Number：
HB-261™
- 物种来源：
小鼠
- 是否是肿瘤细胞：
0
- 生长状态：
悬浮生长
- 运输方式：
冻存运输
| Designations: | FW3-218-1 [218] | ||
| Depositors: | CR Mackay | ||
| Isotype: | IgG2b | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | suspension | ||
| Organism: | Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma) | ||
| Morphology: | lymphoblast |
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| Source: | Cell Type: hybridoma: B lymphocyte; | ||
| Cellular Products: | immunoglobulin; monoclonal antibody; against sheep (ovine) alpha 4 integrin (VLA-4, CD49d) | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Comments: | Animals were immunized with sheep afferent lymph lymphocytes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with and precipitates sheep alpha 4 integrin. It cross-reacts with bovine alpha 4 integrin, but not with the pig, rabbit or human counterparts. |
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| Propagation: | ATCC complete growth medium: Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10% |
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| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
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| Preservation: | Culture medium, 95%; DMSO, 5% | ||
| References: | 22479: Mackay CR, et al. Tissue-specific migration pathways by phenotypically distinct subpopulations of memory T cells. Eur. J. Immunol. 22: 887-895, 1992. PubMed: 1372559 | ||
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文献和实验in Eukaryotic Genomes Reidar Andreson; Lauris Kaplinski; Maido Remm pp. 201 - 218 11. Degenerate Primer Design: Theoretical Analysis and the HYDEN Program Chaim Linhart; Ron Shamir pp. 221 - 244 12. An Iterative Method for Selecting Degenerate
? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 215 Is There a Safe Place to Pause during an RNA Purification Procedure? . . . . . . . . . . . . . . . . . . . . . . . . . . . . 218 What Are the Options to Quantitate Dilute RNA Solutions
with ice-cold PBS at 218 g (1000 rpm, Beckman GS-6 centrifuge, GH-3.8 rotor) at 4oC. 4. After the final PBS wash, resuspend the cells in 5ml of Buffer A and incubate the cells on ice for 5 min. Put a small drop of the cell suspension on a glass slide
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