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        【分享】电子书《PCR Primer Design》

        丁香园论坛

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        【分享】电子书《PCR Primer Design》

        Book Description

        In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as transcriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome.

        In PCR Primer Design, authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome-scale experiments and for small-scale individual PCR amplifications. This volume will be useful for organizations performing whole genome studies, companies designing instruments that utilize PCR, and individual scientists geneticists, molecular biologists, molecular geneticists, and more who routinely use PCR in their research.

        1. Physical Principles and Visual-OMP Software for Optimal PCR Design
        John SantaLucia
        pp. 3 - 34


        2. OLIGO 7 Primer Analysis Software
        Wojciech Rychlik
        pp. 35 - 60


        3. Selection for 3′-End Triplets for Polymerase Chain Reaction Primers
        Kenji Onodera
        pp. 61 - 74


        4. The Reference Point Method in Primer Design
        Thomas Kämpke
        pp. 75 - 92


        5. PCR Primer Design Using Statistical Modeling
        Anton Yuryev
        pp. 93 - 104


        6. Developing a Statistical Model for Primer Design
        Jianping Huang; Anton Yuryev
        pp. 105 - 138


        7. GST-PRIME: An Algorithm for Genome-Wide Primer Design
        Dario Leister; Claudio Varotto
        pp. 141 - 158


        8. Genome-Scale Probe and Primer Design with PRIMEGENS
        Gyan Prakash Srivastava; Dong Xu
        pp. 159 - 176


        9. SNPbox: Web-Based High-Throughput Primer Design with an Eye for Repetitive Sequences
        Stefan Weckx; Peter De Rijk; Wim Glassee; Christine Van Broeckhoven; Jurgen Del-Favero
        pp. 179 - 200


        10. Fast Masking of Repeated Primer Binding Sites in Eukaryotic Genomes
        Reidar Andreson; Lauris Kaplinski; Maido Remm
        pp. 201 - 218

        11. Degenerate Primer Design: Theoretical Analysis and the HYDEN Program
        Chaim Linhart; Ron Shamir
        pp. 221 - 244


        12. An Iterative Method for Selecting Degenerate Multiplex PCR Primers
        Richard Souvenir; Jeremy Buhler; Gary Stormo; Weixiong Zhang
        pp. 245 - 268


        13. Primer Design for Multiplexed Genotyping
        Lars Kaderali
        pp. 269 - 286


        14. MultiPLX: Automatic Grouping and Evaluation of PCR Primers
        Lauris Kaplinski; Maido Remm
        pp. 287 - 304


        15. MultiPrimer: A System for Microarray PCR Primer Design
        Rohan Fernandes; Steven Skiena
        pp. 305 - 314


        16. Modified Oligonucleotides as Tools for Allele-Specific Amplification
        Michael Strerath; Ilka Detmer; Jens Gaster; Andreas Marx
        pp. 317 - 328


        17. AlleleID: A Pathogen Detection and Identification System
        Arun Apte; Siddharth Singh
        pp. 329 - 346


        18. Designing Primers for Whole Genome PCR Scanning Using the Software Package GenoFrag: A Software Package for the Design of Primers Dedicated to Whole-Genome Scanning by LR-PCR
        Nouri Ben Zakour; Yves Le Loir
        pp. 349 - 368


        19. Designing PCR Primer for DNA Methylation Mapping
        Long-Cheng Li
        pp. 371 - 384


        20. BiSearch: ePCR Tool for Native or Bisulfite-Treated Genomic Template
        Tamás Arányi; Gábor E. Tusnády
        pp. 385 - 402

        21. Graphical Design of Primers with PerlPrimer
        Owen Marshall
        pp. 403 - 414

        http://rapidshare.com/files/83213261/pcprides.rar
        http://www.filefactory.com/file/0560b3/
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