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- 文献和实验
- 技术资料
- 库存:
大量
- 生长状态:
贴壁生长
- 器官来源:
其他
- 相关疾病:
正常
- ATCC Number:
CRL-2642™
- 年限:
1 to 6 days posthatching juvenile
- 运输方式:
冻存运输
- 物种来源:
其他
- 是否是肿瘤细胞:
0
- 细胞形态:
成纤维样
| Designations: | Fugu fry | ||
| Depositors: | DW Barnes | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Fugu niphobles | ||
| Morphology: | fibroblast |
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| Source: | Organ: fry, whole Disease: normal |
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| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Isolation: | Isolation date: 1996 | ||
| Cytogenetic Analysis: | hyperploid | ||
| Age: | 1 to 6 days posthatching juvenile | ||
| Comments: | The Fugu fry cell line was established in 1996 from normal juvenile whole fry. Fugu cells maintain an unusually compact genome size with small introns. They are telomerase positive. [53446 ] The cell line may be used for in vitro vertebrate genome studies, and in marine fish and aquaculture research. |
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| Propagation: | ATCC complete growth medium: 67.5 % DMEM HG without sodium bicarbonate (GIBCO Cat. # 12100), 25 % L-15 without sodium bicarbonate (Vitacell 30-2008), 7.5 % Ham's F12 without sodium bicarbonate (GIBCO Cat. # 21700) Supplemented with: 15 mM HEPES, 0.5 g/L sodium bicarbonate, 0.01 mg/ml insulin, 0.05 mM 2-mercaptoethanol, 1.0 mM L-glutamine, 0.05 mM non-essential Amino Acids, 10 ng/ml basic human recombinant FGF, 50 ng/ml mouse EGF (Do not filter), 5 to 7.5% heat-inactivated fetal bovine serum Temperature: 22.0°C; (Max. 23.0 °C, Min. 21.0°C) |
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| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended; cell density must be high. Medium Renewal: Every 2 to 3 days |
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| Preservation: | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
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| Doubling Time: | 2.5 days | ||
| References: | 53446: Bradford CS, et al. Characterization of cell cultures derived from Fugu, the Japanese pufferfish. Mol. Mar. Biol. Biotechnol. 6: 279-288, 1997. PubMed: 9418286 | ||
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文献和实验脊椎动物,鱼纲,�科,东方�属鱼类的通称。又称河豚。主要生活于海洋的中、下层,有些种也进入淡水。我国沿海均产。体长约30厘米,呈圆筒形,口小,牙愈合成牙板,常露出吻外。背鳍1个,与臀鳍对生,无腹鳍。无鳞或有刺鳞。有气囊,遇敌时能够吸气膨胀,呈球状,使腹部朝向水面装死,故俗称“气泡鱼”。肉极鲜美,唯肝脏、生殖腺、血液和皮肤含有河�毒素,需经仔细处理后,方可食用,否则,食后可引起中毒,甚至死亡。可制成冻鱼片和罐头食品。皮可制鱼皮胶,骨可制鱼粉,卵巢中的河�毒素可用作制药原料。成熟
脊椎动物,鱼纲,鲀科,东方鲀属鱼类的通称。又称河豚。主要生活于海洋的中、下层,有些种也进入淡水。我国沿海均产。体长约 30厘米,呈圆筒形,口小,牙愈合成牙板,常露出吻外。背鳍 1个,与臀鳍对生,无腹鳍。无鳞或有刺鳞。有气囊,遇敌时能够吸气膨胀,呈球状,使腹部朝向水面装死,故俗称“气泡鱼”。肉极鲜美,唯肝脏、生殖腺、血液和皮肤含有河鲀毒素,需经仔细处理后,方可食用,否则,食后可引起中毒,甚至死亡。可制成冻鱼片和罐头食品。皮可制鱼皮胶,骨可制鱼粉,卵巢中的河鲀毒素可用作制药原料
主要是指河鲀的卵巢和肝脏中所含的毒素,尤其是供食用的河鲀,毒性更烈。圆鲀、扁背鲀等几乎没有毒性。虽认为在皮肤,肠等里面也存在着毒性,但血液几乎无毒,毒性的有效成分是河豚毒素( tetro-dotoxin)其结构式如图所示,游离碱难溶于水和有机溶剂,与酸形成盐时,就成为水溶性。水溶液在微酸性下稳定,在碱性中不稳定。对小鼠的半数致死量为 0.6— 8.5微克 /公斤体重。 河豚中毒的致死是由于呼吸麻痹引起的,另外还有肌肉弛缓,感觉
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