Fugu fry产品图

Fugu fry

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  • 询价
  • 2026年08月18日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      大量

    • 生长状态

      贴壁生长

    • 器官来源

      其他

    • 相关疾病

      正常

    • ATCC Number

      CRL-2642™

    • 年限

      1 to 6 days posthatching juvenile

    • 运输方式

      冻存运输

    • 物种来源

      其他

    • 是否是肿瘤细胞

      0

    • 细胞形态

      成纤维样

    Designations: Fugu fry
    Depositors:  DW Barnes
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Fugu niphobles
    Morphology: fibroblast

    Source: Organ: fry, whole
    Disease: normal
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Isolation: Isolation date: 1996
    Cytogenetic Analysis: hyperploid
    Age: 1 to 6 days posthatching juvenile
    Comments: The Fugu fry cell line was established in 1996 from normal juvenile whole fry.
    Fugu cells maintain an unusually compact genome size with small introns.
    They are telomerase positive. [53446 ]
    The cell line may be used for in vitro vertebrate genome studies, and in marine fish and aquaculture research.
    Propagation: ATCC complete growth medium: 67.5 % DMEM HG without sodium bicarbonate (GIBCO Cat. # 12100), 25 % L-15 without sodium bicarbonate (Vitacell 30-2008), 7.5 % Ham's F12 without sodium bicarbonate (GIBCO Cat. # 21700) Supplemented with: 15 mM HEPES, 0.5 g/L sodium bicarbonate, 0.01 mg/ml insulin, 0.05 mM 2-mercaptoethanol, 1.0 mM L-glutamine, 0.05 mM non-essential Amino Acids, 10 ng/ml basic human recombinant FGF, 50 ng/ml mouse EGF (Do not filter), 5 to 7.5% heat-inactivated fetal bovine serum
    Temperature: 22.0°C; (Max. 23.0 °C, Min. 21.0°C)
    Subculturing: Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 21 to 23�C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.
    6. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
    7. Incubate cultures at 21 to 23�C without CO2.

    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended; cell density must be high.
    Medium Renewal: Every 2 to 3 days
    Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Doubling Time: 2.5 days
    References: 53446: Bradford CS, et al. Characterization of cell cultures derived from Fugu, the Japanese pufferfish. Mol. Mar. Biol. Biotechnol. 6: 279-288, 1997. PubMed: 9418286

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    图标文献和实验
    相关实验
    • 河(Fugu

        脊椎动物,鱼纲,�科,东方�属鱼类的通称。又称河豚。主要生活于海洋的中、下层,有些种也进入淡水。我国沿海均产。体长约30厘米,呈圆筒形,口小,牙愈合成牙板,常露出吻外。背鳍1个,与臀鳍对生,无腹鳍。无鳞或有刺鳞。有气囊,遇敌时能够吸气膨胀,呈球状,使腹部朝向水面装死,故俗称“气泡鱼”。肉极鲜美,唯肝脏、生殖腺、血液和皮肤含有河�毒素,需经仔细处理后,方可食用,否则,食后可引起中毒,甚至死亡。可制成冻鱼片和罐头食品。皮可制鱼皮胶,骨可制鱼粉,卵巢中的河�毒素可用作制药原料。成熟

    • 河鲀(Fugu

        脊椎动物,鱼纲,鲀科,东方鲀属鱼类的通称。又称河豚。主要生活于海洋的中、下层,有些种也进入淡水。我国沿海均产。体长约 30厘米,呈圆筒形,口小,牙愈合成牙板,常露出吻外。背鳍 1个,与臀鳍对生,无腹鳍。无鳞或有刺鳞。有气囊,遇敌时能够吸气膨胀,呈球状,使腹部朝向水面装死,故俗称“气泡鱼”。肉极鲜美,唯肝脏、生殖腺、血液和皮肤含有河鲀毒素,需经仔细处理后,方可食用,否则,食后可引起中毒,甚至死亡。可制成冻鱼片和罐头食品。皮可制鱼皮胶,骨可制鱼粉,卵巢中的河鲀毒素可用作制药原料

    • 河鲀毒fugu toxin

          主要是指河鲀的卵巢和肝脏中所含的毒素,尤其是供食用的河鲀,毒性更烈。圆鲀、扁背鲀等几乎没有毒性。虽认为在皮肤,肠等里面也存在着毒性,但血液几乎无毒,毒性的有效成分是河豚毒素( tetro-dotoxin)其结构式如图所示,游离碱难溶于水和有机溶剂,与酸形成盐时,就成为水溶性。水溶液在微酸性下稳定,在碱性中不稳定。对小鼠的半数致死量为 0.6— 8.5微克 /公斤体重。   河豚中毒的致死是由于呼吸麻痹引起的,另外还有肌肉弛缓,感觉

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