Fugu eye产品图

Fugu eye

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  • 询价
  • 2026年08月18日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 器官来源

    • 生长状态

      贴壁生长

    • 物种来源

      其他

    • 是否是肿瘤细胞

      0

    • 运输方式

      冻存运输

    • 年限

      juvenile juvenile

    • 细胞形态

      上皮样

    • 相关疾病

      正常

    • ATCC Number

      CRL-2641™

    • 库存

      大量

    Designations: Fugu eye
    Depositors:  DW Barnes
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Fugu rubripes
    Morphology: epithelial

    Source: Organ: eye
    Disease: normal
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Isolation: Isolation date: 1996
    Cytogenetic Analysis: diploid
    Age: juvenile juvenile
    Comments: The Fugu eye cell line was established in 1996 from normal eye tissue.
    Fugu cells maintain an unusually compact genome size with small introns.
    They are telomerase positive. [53446 ]
    The cell line may be used for in vitro vertebrate genome studies, and in marine fish and aquaculture research.
    Propagation: ATCC complete growth medium: 67.5 % DMEM HG without sodium bicarbonate (GIBCO Cat. # 12100), 25 % L-15 without sodium bicarbonate (Vitacell 30-2008), 7.5 % Ham's F12 without sodium bicarbonate (GIBCO Cat. # 21700) Supplemented with: 15 mM HEPES, 0.5 g/L sodium bicarbonate, 0.01 mg/ml insulin, 0.05 mM 2-mercaptoethanol, 1.0 mM L-glutamine, 0.05 mM non-essential Amino Acids, 10 ng/ml basic human recombinant FGF, 50 ng/ml mouse EGF (Do not filter), 5 to 7.5% heat-inactivated fetal bovine serum
    Temperature: 22.0°C; (Max. 23.0 °C, Min. 21.0°C)
    Subculturing: Protocol:
    1. Remove and discard culture medium.
    2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
    3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
      Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 21 to 23�C to facilitate dispersal.
    4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
    5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.
    6. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
    7. Incubate cultures at 21 to 23�C without CO2.

    Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended; cell density must be high.
    Medium Renewal: Every 2 to 3 days
    Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Doubling Time: 2.5 days
    References: 53446: Bradford CS, et al. Characterization of cell cultures derived from Fugu, the Japanese pufferfish. Mol. Mar. Biol. Biotechnol. 6: 279-288, 1997. PubMed: 9418286

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    图标文献和实验
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    • 复眼 compound eye,facet-eye

      特殊结构的透镜眼。由很多个小眼集合成蜂巢状的眼。节肢动物(昆虫类、甲壳类、剑尾类、唇足类)通常具有一对。此外多毛类和斧足类等也有复眼。昆虫类在成虫或若虫开始出现,但穴栖昆虫和职蚁则缺如。组成一个复眼的小眼数目,蚁Solenopsis的职蚁(营地下生活)6—9个,家蝇4000个,萤(Lamp-yris)雄虫(有翅)2500个雌虫(无翅)300个,具缘龙9000个、日本粉鳃金龟子5100个、蜻蜒1万—2.8万个。小眼的外面(小眼面)一般呈六角形成五角形,密集排列,但数目少时则为圆形或近于圆形

    • 眼(eye

         眼是视觉器官。外界物体的光线射入眼中,聚焦后在视网膜上成像,光能在视网膜内转变成神经冲动,经视神经传至大脑皮层视区,产生视觉。人眼近似球形,称眼球,前后极距离约 24毫米,后面有视神经连于脑。眼球由眼球壁及内部的折光物质所构成。   眼球壁 由外膜、中膜及内膜组成。   外膜 也称纤维膜。由致密结缔组织构成,强韧而厚,具有保护作用。其前 1/6为曲度较大而透明的角膜( cornea),对光线的聚焦起主要作用,角膜

    • 河(Fugu

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