![CHO DP-12 clone#1934 [CHO DP-12, clone#1934 aIL8.92 NB 28605/14]产品图](https://custom.dxycdn.com/trademd/upload/pic/2012/03/29/1332499969.jpg!small.t.1)
- 询价
- 2026年08月15日
15 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
从原料到标记，打造属于中国自己的流式抗体品牌Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 物种来源：
仓鼠
- 是否是肿瘤细胞：
0
- 库存：
大量
- 免疫类型：
human IgG1
- 细胞形态：
成纤维样
- 器官来源：
卵巢
- 运输方式：
冻存运输
- 生长状态：
贴壁生长
- ATCC Number：
CRL-12445™
| Designations: | CHO DP-12 clone#1934 [CHO DP-12, clone#1934 aIL8.92 NB 28605/14] | ||
| Depositors: | Genetech, Inc. | ||
| Isotype: | human IgG1 | ||
| Biosafety Level: | 1 | ||
| Shipped: | frozen | ||
| Medium & Serum: | See Propagation | ||
| Growth Properties: | adherent | ||
| Organism: | Cricetulus griseus | ||
| Morphology: | fibroblast |
||
| Source: | Organ: ovary | ||
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | ||
| Applications: | The cells are reported to produce 250 mg/L recombinant human anti-IL-8. Both the clone#1933 (ATCC -CRL-12444) and clone#1934 (ATCC CRL-12445 ) antibodies inhibit IL-8 binding to human neutrophils. The clone#1934 aIL8.42 NB 28605/14 cell line was derived by co-transfecting the CHO cell line DP-12 using Lipofection with the vector p6G4V11N35E.choSD.10 designed to coexpress variable light and heavy regions of the murine 6G4.2.5 monoclonal antibody (ATCC -HB-11722). |
||
| Comments: | The clone#1934 aIL8.42 NB 28605/14 cell line was derived by co-transfecting the CHO cell line DP-12 using Lipofection with the vector p6G4V11N35E.choSD.10 designed to coexpress variable light and heavy regions of the murine 6G4.2.5 monoclonal antibody (ATCC -HB-11722). Clones were selected in methotrexate. The cells are reported to produce 250 mg/L recombinant human anti-IL-8. Both the clone#1933 (ATCC -CRL-12444) and clone#1934 (ATCC CRL-12445 ) antibodies inhibit IL-8 binding to human neutrophils. They also show equivalent neutralizing capabilities to inhibiting IL-8 mediated human neutrophil chemotaxis.The mammalian expression plasmid, p6G4V11N35AchoSD.9 (identified as p6G425V11N35A.choSD is deposited as ATCC 209552 ). | ||
| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 200 nM MTX (Methotrexate), Trace elements A and B from Mediatech, 0.002 mg/ml rhInsulin and 10% fetal bovine serum. Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
||
| Subculturing: | Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Two to three times weekly |
||
| Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
||
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002 recommended serum:ATCC 30-2020 |
||
| References: | 71467: Hsei V, et al. Methods of treating inflammatory diseases with anti-IL-8 antibody fragment-polymer conjugates. US Patent 6,468,532 dated Oct 22 2002 71468: Hsei V, et al. Methods of treating inflammatory disease with anti-IL-8 antibody fragment-polymer conjugates. US Patent 6,458,355 dated Oct 1 2002 71469: Gonzalez TN, et al. Humanized anti-IL-8 monoclonal antibodies. US Patent 6,133,426 dated Oct 17 2000 71470: Gonzalez TN, et al. Humanized anti-IL-8 monoclonal antibodies. US Patent 6,117,980 dated Sep 12 2000 71471: Gonzalez TN, et al. Nucleic acids encoding humanized anti-IL-8 monoclonal antibodies. US Patent 6,025,158 dated Feb 15 2000 |
||
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
Establishment of Stable Transfectant of CHO Lec Cells
-deoxynojirimycin (NB-DNJ), glycoproteins produced in CHO Lec 3.2.8.1 cells are almost completely susceptible to Endo H digestion (Davis, 1995; Ikemizu, 1999). Endo H cleaves chitobiose, leaving a single N-linked N-acetylglucosamine per site, which is ideal
Establishment of Stable Transfectant of CHO Lec Cells
. When cultured in the presence of the alpha-glucosidase I inhibitor N-butyl-deoxynojirimycin (NB-DNJ), glycoproteins produced in CHO Lec 3.2.8.1 cells are almost completely susceptible to Endo H digestion (Davis, 1995; Ikemizu, 1999). Endo H cleaves chitobiose
|11595616|emb|AL451018.1|NC99H12 Neurospora crassa DNA li... 32 42 gi|12718469|emb|AL513466.1|NCB18D24 Neurospora crassa DNA l... 32 42 gi|13346588|gb|AC020601.10| Homo sapiens BAC clone RP11-451... 32 42 gi|31616680|emb|AL837516.14| Mouse DNA sequence
技术资料暂无技术资料 索取技术资料
CHO DP-12 clone#1934 [CHO DP-12, clone#1934 aIL8.92 NB 28605/14]
询价
