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Hieff NGS® Fast Tagment DNA Library Prep Kit for Illumina® (for50 ng)
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有效期1年
翌圣生物科技(上海)股份有限公司
-20℃保存
8 T
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产品描述Hieff NGS® Fast Tagment DNA Library Prep Kit for Illumina® (for 50 ng)是针对lllumina®高通量测序平台专业开发设计的转座酶法建库试剂盒,适用于50 ng的基因组DNA、cDNA、扩增子 (>500 bp)等样本的建库。与常规分步法建库相比,Hieff NGS® Fast Tagment DNA Library Prep Kit for Illumina®采用新型转座酶和优化的缓冲体系,仅需10 min即可完成DNA片段化、末端修复和接头连接过程,显著缩短建库时间至1.5小时以内,并获得优异的测序质量。此外,本试剂盒已经不同GC含量DNA样本的验证,无偏好性或仅具有极低的偏好性。本试剂盒提供的所有试剂盒组分,都经过严格的质量与功能验证,最高程度保障产品优异性能与批间稳定性
组分编号 | 组分名称 | 8 T | 24 T | 96 T |
12207-A | 5×Reaction Buffer | 80 μL | 240 μL | 960 μL |
12207-B | Transposome Mix V50 | 40 μL | 120 μL | 480 μL |
12207-C | 6×Terminate Solution | 80 μL | 240 μL | 960 μL |
12207-D | PCR Primer Mix | 24 μL | 72 μL | 288 μL |
12207-E | 2×Ultima Amplification Mix | 200 μL | 600 μL | 2400 μL |
12207-F | N5 (N501)* | 8 μL | - | - |
12207-G | N7 (N701)* | 4 μL | - | - |
12207-H | N7 (N702)* | 4 μL | - | - |
名称 | 体积 (μL) |
50 ng Input DNA | x |
5×Reaction Buffer | 10 |
Transposome Mix V50 | 5 |
ddH2O | Up to 50 μL |
温度 | 时间 |
热盖105°C | On |
55°C | 10 min |
4°C | Hold |
温度 | 时间 |
热盖105°C | On |
55°C | 10 min |
4°C | Hold |
名称 | 体积 (μL) |
步骤三纯化产物 | 20 |
PCR Primer Mix | 3 |
N5XX* | 1 |
N7XX* | 1 |
2×Ultima Amplification Mix | 25 |
ddH2O | Up to 50 μL |
温度 | 时间 | 循环数 |
热盖105°C | On | - |
72°C | 3 min* | 1 |
95°C | 30 sec | 1 |
95°C | 10 sec | n** |
55°C | 30 sec | |
72°C | 30 sec | |
72°C | 5 min | 1 |
4°C | Hold | - |
文库平均总长度 | ~300 bp | ~400 bp | ~500 bp | ~800 bp |
第一轮磁珠用量 | 80 μL (0.8×) | 70 μL (0.7×) | 60 μL (0.6×) | 50 μL (0.5×) |
第二轮磁珠用量 | 20 μL (0.2×) | 20 μL (0.2×) | 20 μL (0.2×) | 15 μL (0.15×) |
建库试剂盒 | 产品编号 | 规格 | 价格(元) |
Hieff NGS® MaxUp Dual-mode mRNA Library Prep Kit for Illumina® | 12301ES24/96 | 24/96 T | 9756/32856 |
Hieff NGS® OnePot DNA Library Prep Kit for Illumina® | 12203ES24/96 | 24/96 T | 7486/28567 |
Hieff NGS® MaxUpⅡ DNA Library Prep Kit for Illumina® | 12200ES24/96 | 24/96T | 6486/23567 |
Hieff NGS® MaxUp DNA Library Prep Kit for Illumina® | 12201ES24/96 | 24/96T | 4626/16626 |
Hieff NGS® Fast Tagment DNA Library Prep Kit for Illumina® | 12206ES24/96 | 24/96 T | 6155/23855 |
文库构建磁珠 | 产品编号 | 规格 | 价格(元) |
Hieff NGS® cfDNA Clean Beads(100~200 bp) | 12599ES08/56 | 5/60 mL | 1666/9106 |
Hieff NGS® Smarter DNA Clean beads (50 bp以上) | 12600ES08/56 | 5/60 mL | 1386/7586 |
Hieff NGS® DNA Selection Beads(Superior AMPure XP alternative) | 12601ES08/56 | 5/60 mL | 986/6286 |
Hieff NGS® RNA Cleaner | 12602ES08/56 | 5/60 mL | 1066/4266 |
Hieff NGS® mRNA Isolation Master Kit | 12603ES24/96 | 24/96 T | 616/2266 |
建库接头 | 产品编号 | 规格 | 价格(元) |
Hieff NGS® Complete Adapter Kit for Illumina®, Set 1 | 12615ES04/16 | 12×4/12×16 T | 1256/4536 |
Hieff NGS® Complete Adapter Kit for Illumina®, Set 2 | 12616ES04/16 | 12×4/12×16 T | 1256/4536 |
Hieff NGS® Complete Adapter Kit for Illumina®, Set 3 | 12617ES04/16 | 12×4/12×16 T | 1256/4536 |
Hieff NGS® Complete Adapter Kit for Illumina®, Set 4 | 12618ES04/16 | 12×4/12×16 T | 1256/4536 |
Hieff NGS® Tagment Index Kit for Illumina®,(96 Index) | 12610ES96 | 96 T | 1365 |
建库模块 | 产品编号 | 规格 | 价格(元) |
Hieff NGS® Fast-Pace DNA Fragmentation Reagent | 12609ES50 | 50 T | 1466 |
Hieff NGS® Fast-Pace End Repair/dA-Tailing Module | 12608ES24/96 | 24/96 T | 2066/6166 |
2×Super Canace®Ⅱ High-Fidelity Mix for Library Amplification | 12621ES03/08 | 1/5×1mL | 725/1855 |
Hieff NGS® Dual-Mode cDNA Synthesis Kit | 12250ES24/96 | 24/96 T | 4885/16485 |
文库定量 | 产品编号 | 规格 | 价格(元) |
Hieff NGS® Library Quantification Kit for Illumina®, qPCR Master Mix | 12302ES05 | 500 T | 1526 |
Hieff NGS® Library Quantification Kit for Illumina®, DNA Standard (1-6) | 12307ES09 | 6×96 μL | 2126 |
dsDNA HS Assay Kit for Qubit® | 12640ES60/76 | 100/500 T | 686/2696 |
多重PCR | 产品编号 | 规格 | 价格(元) |
Hieff® Multiplex PCR Kit | 12279ES50/76 | 100/500 T | 875/3165 |
Hieff® Multiplex PCR Master Mix | 12280ES50/76 | 50 /200 T | 875/3165 |
HB210809
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12207-DNA转座酶法建库试剂盒.pdf 附 (下载 3 次)