JAK1 单克隆抗体 66466-1-Ig产品图
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JAK1 单克隆抗体 66466-1-Ig

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  • ¥1350 - 3000
  • Proteintech已认证
  • 66466-1-Ig
  • 2025年10月13日
  • WB, IHC, ELISA
  • Mouse
  • human, rat, mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Recombinant human JAK1 protein

    • 亚型

      IgG2b

    • 保存条件

      Store at -20°C. Stable for one year after shipment.

    • 克隆性

      Monoclonal

    • 标记物

      Unconjugated

    • 适应物种

      human, rat, mouse

    • 保质期

      详见产品包装

    • 库存

      充足

    • 宿主

      Mouse

    • 应用范围

      WB, IHC, ELISA

    • 靶点

      JAK1

    • 抗体英文名

      JAK1 Monoclonal antibody

    • 抗体名

      JAK1 Monoclonal antibody

    • 规格

      50ul/100ul/150ul

    规格:50ul产品价格:¥1350.0
    规格:100ul产品价格:¥2200.0
    规格:150ul产品价格:¥3000.0

    经过测试的应用

    Positive WB detected inK-562 cells, Jurkat cells, rat spleen tissue, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells, Ramos cells, Daudi cells
    Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
    Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
    Planning an IHC experiment? We recommend our IHCeasy JAK1 Ready-To-Use IHC Kit. JAK1 primary antibody included.

    推荐稀释比

    应用推荐稀释比
    Western Blot (WB)WB : 1:1000-1:6000
    Immunohistochemistry (IHC)IHC : 1:100-1:400
    It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
    Sample-dependent, Check data in validation data gallery.

    产品信息

    66466-1-Ig targets JAK1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, rat, mouse samples.

    经测试应用 WB, IHC, ELISA
    文献引用应用WB, IHC, IF, IP
    经测试反应性 human, rat, mouse
    文献引用反应性human, mouse, rat
    免疫原

    CatNo: Ag17940

    Product name: Recombinant human JAK1 protein

    Source: e coli.-derived, PGEX-4T

    Tag: GST

    Domain: 8-363 aa of BC132729

    Sequence: EDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEW

    宿主/亚型 Mouse / IgG2b
    抗体类别 Monoclonal
    产品类型 Antibody
    全称 Janus kinase 1 (a protein tyrosine kinase)
    别名 JAK 1, JAK1, JAK1A, JAK1B, Janus kinase 1, JTK3, Tyrosine protein kinase JAK1
    计算分子量 1154 aa, 133 kDa
    观测分子量 133 kDa
    GenBank蛋白编号BC132729
    基因名称 JAK1
    Gene ID (NCBI) 3716
    RRIDAB_2881834
    偶联类型 Unconjugated
    形式Liquid
    纯化方式Protein A purification
    UNIPROT IDP23458
    储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
    储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

    背景介绍

    Janus kinase 1(JAK1), is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. JAK1 is essential for signaling for certain type I and type II cytokines. JAK1 plays a critical role in initiating responses to multiple major cytokine receptor families. Expression of JAK1 in cancer cells enables individual cells to contract, potentially allowing them to escape their tumor and metastasize to other parts of the body.

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    图标文献和实验
    该产品被引用文献
    [1]Natural flavonoid dihydroquercetin blocks Trim14-JAK1-STAT3 signaling and relieves pain-depression comorbidity in rheumatoid arthritis
    PMID:42393024Transl Psychiatry2026-07-01
    [2]Dimethyl itaconate suppresses dendritic cell and CD8+ T cell responses to halt vitiligo.
    PMID:41466279BMC Med2025-12-29
    [3]Taurine Attenuates M1 Macrophage Polarization and IL-1β Production by Suppressing the JAK1/2-STAT1 Pathway via Metabolic Reprogramming.
    PMID:41463524Biology (Basel)2025-12-06
    [4]Oncostatin M mediates the causal relationship between obesity and chronic kidney disease: evidence from Mendelian randomization and experimental validation.
    PMID:41340158Diabetol Metab Syndr2025-12-03
    [5]Modified Sijunzi Decoction Inhibits Pancreatic Cancer Progression and Improves Immune Suppression: Network Pharmacology and Experimental Validation.
    PMID:41058947Drug Des Devel Ther2025-10-19
    [6]Fecal Microbiota Transplantation Modulates Th17/Treg Balance via JAK/STAT Pathway in ARDS Rats.
    PMID:40575995Adv Biol (Weinh)2025-09
    [7]ADRB2 is regulated by TRIM22 and facilitates lung adenocarcinoma progression via JAK2/STAT3 signaling pathway.
    PMID:40594822Sci Rep2025-07-01
    [8]Proteome-wide ligandability maps of drugs with diverse cysteine-reactive chemotypes.
    PMID:40419519Nat Commun2025-05-26
    [9]Suberosin attenuates rheumatoid arthritis by repolarizing macrophages and inhibiting synovitis via the JAK/STAT signaling pathway
    PMID:39838477Arthritis Res Ther2025-01-21
    [10]Exosomes derived from syncytia induced by SARS-2-S promote the proliferation and metastasis of hepatocellular carcinoma cells.
    PMID:39844837Front Cell Infect Microbiol2025-01-08
    相关实验
    • 单克隆抗体制备

      抗体的检测外,还应该用与其抗原成分相关的其它抗原进行交叉试验,方法可用ELISA、IFA法。例如:①制备抗黑色素瘤细胞的McAb,除用黑色素瘤细胞反应外,还应该用其它脏器的肿瘤细胞和正常细胞进行交叉反应,以便挑选肿瘤特异性或肿瘤相关抗原的单克隆抗体。②制备抗重组的细胞因子的单克隆抗体,应首先考虑是否与表达菌株的蛋白有交叉反应,其次是与其它细胞因子间有无交叉。 2.McAb的Ig类与亚类的鉴定   一般在用酶标或荧光素标记的第二抗体进行筛选时已经基本上确定了抗体的Ig类型

    • 免疫球蛋白(immunoglobulim,Ig)

      免疫系统受抗原刺激后,B细胞转化为浆细胞,由浆细胞产生与抗原发生特异性结合的球蛋白,这类球蛋白称为抗体(antibody,Ab)。 免疫球蛋白(immunoglobulim,Ig)是指具有抗体活性或化学结构与抗体相似的球蛋白。 免疫球蛋白普遍存在于哺乳动物和人类的血液、组织液和外分泌中。人体的免疫球蛋白主要有五类:IgM、IgA、IgG、IgD、IgE。 (一) 抗体结构 抗体是具有4条多肽链的对称

    • 单克隆抗体的生产

      浓度很高。可见该法操作简便、经济,不过,腹水中常混有小鼠的各种杂蛋白(包括Ig),因此在很多情况下要提纯后才能使用,而且还有污染动物病毒的危险,故而最好用SPF级小鼠。(1)材料:a. 成年BALB/c小鼠。b. 降植烷(Pristane)或液体石蜡。c. 处于对数生长期的杂交瘤细胞。(2)方法:a. 腹腔接种降植烷或液体石蜡,每只小鼠0.3-0.5ml。b. 7-10天后腹腔接种用PBS或无血清培养基稀释的杂交瘤细胞,每只小鼠5×105/0.2ml。c. 间隔5天后,每天观察小鼠腹水产生情况,如腹部

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    JAK1 单克隆抗体 66466-1-Ig
    ¥1350 - 3000