- ￥1350 - 3000
- Proteintech
- 60070-1-Ig
- 2025年10月13日
- WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Pre IL-18 protein
- 亚型：
IgG2b
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Mouse
- 应用范围：
WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
- 靶点：
IL-18
- 抗体英文名：
IL-18 Monoclonal antibody
- 抗体名：
IL-18 Monoclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | U2OS cells, HeLa cells, A549 cells, HepG2 cells |
| Positive IHC detected in | human tonsillitis tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60070-1-Ig targets IL-18 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA |
| 文献引用应用 | WB, IHC, IF, ELISA |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag1063 Product name: Recombinant human Pre IL-18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-193 aa of BC007461 Sequence: MAAEPVEDNCINFVAMKFIDNTLYFIAEDDENLESDYFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | IL-18 |
| 别名 | IL18, Pre-IL18, 5C6F8, Iboctadekin, IFN-gamma-inducing factor |
| 计算分子量 | 193 aa, 22 kDa |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | BC007461 |
| 基因名称 | IL-18 |
| Gene ID (NCBI) | 3606 |
| RRID | AB_2280158 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q14116 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IL-18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form.
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Nature Recognition and maturation of IL-18 by caspase-4 noncanonical inflammasomeAuthors - Xuyan Shi |
| WB | Stem Cells Transl Med Mesenchymal Stem Cells-Derived Exosomes Alleviate Acute Lung Injury By Inhibiting Alveolar Macrophage PyroptosisAuthors - Peipei Liu | |
| WB | J Neuroinflammation GPR120 modulates epileptic seizure and neuroinflammation mediated by NLRP3 inflammasome.Authors - Zhangjin Qin | |
| WB | Int Immunopharmacol Long non-coding RNA NEAT1 exacerbates NLRP3-mediated pyroptosis in allergic rhinitis through regulating the PTBP1/FOXP1 cascadeAuthors - Yunliang Liu | |
| rat | WB | J Ethnopharmacol Neuroprotective effects of Jie-du-huo-xue decoction on microglia pyroptosis after cerebral ischemia and Reperfusion--From the perspective of glial-vascular unitAuthors - Chang Zhou |
| rat | WB | J Inflamm (Lond) Carvacrol alleviates LPS-induced myocardial dysfunction by inhibiting the TLR4/MyD88/NF-κB and NLRP3 inflammasome in cardiomyocytesAuthors - Lu Xu |
49e/CD29、CD49f/CD29、CD51/CD29、CD11a/CD18、CD11b/CD18、CD11c/CD18、CD41/CD61、CD51/CD61、CD49f/CD104、CD2、CD4、CD8、CD28、CD31、CD50、CD54、CD56、CD58、CD80、CD102、CD106、CD62L、CD62P、CD62E、CD15、CD15s、CD44等。 （2）B表示CD命名范围中属IGSF结构的分子，如CD79a、CD79b、CD1、CD
5等是嗜酸性粒细胞发挥ADCC效应的有效激活剂，在抗寄生虫和抗肿瘤中有重要作用。GM-CSF激活作用主要是通过FcγRⅡ介导的。 （4）NK细胞：通过FcγRⅢA介导ADCC杀伤肿瘤细胞等靶细胞，IL-2和IFN-γ可明显提高NK细胞的杀伤活性，但并不明显改变FcγRⅢ的表达水平。 （二）FcαR FcαR（CD89）为分子量60kD的穿膜糖蛋白，属Ig超家族，胞膜外有2个C2功能区，呈中等亲和力，主要表达于单核细胞、巨噬细胞、中性粒细胞等，介导吞噬，ADCC以及炎症介质的释放
CD18 分子 CD18 常用单克隆抗体或代号： MHM23;(LFA组b 链,integrin b 2,CR3) 主要表达细胞： Leu [AS] 分子质量(kDa)和结构： gp95 功 能： ICAM- 1(CD54)、ICAM- 2(CD102)、ICAM- 3、C3bi配体,粘附,调理吞噬 CD18 Forms the beta 2 chain of CD11a, b, c
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