- ￥1350 - 3000
- Proteintech
- 21990-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human ANXA1 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
- 靶点：
Annexin A1
- 抗体英文名：
Annexin A1 Polyclonal antibody
- 抗体名：
Annexin A1 Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A549 cells, A431 cells, C6 cells, HeLa cells, HepG2 cells, MCF-7 cells, MDA-MB-453s cells, multi-cells, U-937 cells, K-562 cells, NIH/3T3 cells, Calu-1 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon tissue, human kidney tissue, human lung tissue, human oesophagus tissue, human ovary cancer tissue, human ovary tumor tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | NIH/3T3 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21990-1-AP targets Annexin A1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, bovine |
| 免疫原 |
CatNo: Ag17273 Product name: Recombinant human ANXA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-346 aa of BC001275 Sequence: MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | annexin A1 |
| 别名 | ANXA1, Annexin 1, Annexin Ac2-26, Annexin I, Annexin-1 |
| 计算分子量 | 346 aa, 39 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC001275 |
| 基因名称 | Annexin A1 |
| Gene ID (NCBI) | 301 |
| RRID | AB_11182596 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P04083 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB,IF | Nature Evolutionarily divergent mTOR remodels translatome for tissue regenerationAuthors - Olena Zhulyn |
| IP | Cell Host Microbe Global mapping of Salmonella enterica-host protein-protein interactions during infection.Authors - Philipp Walch | |
| human | WB | Oncogene The E3 ligase RBCK1 reduces the sensitivity of ccRCC to sunitinib through the ANKRD35-MITD1-ANXA1 axisAuthors - Yapeng Wang
|
| human | WB,IP,IHC,IF | Elife ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein.Authors - Horia Vais |
| human | WB | Mol Ther Membrane Stabilization by Modified Steroid Offers a Potential Therapy for Muscular Dystrophy Due to Dysferlin Deficit.Authors - Sen Chandra Sreetama |
| human | WB | Cancer Immunol Immunother Circulating PD-L1 is associated with T cell infiltration and predicts prognosis in patients with CRLM following hepatic resection.Authors - Xiuxing Chen |
Annexin V-PI 双染法 在细胞凋亡的早期就有细胞膜表面破损发生。破损时凋亡细胞表面的磷脂腺丝氨酸(PS)可以从细胞内膜翻转至细胞的外膜。该过程发生在DNA片段化之前，因而检测PS的表达，能反映早期凋亡。AnnexinV是相对分子质量为35 000大小的Ca2+依赖的磷脂结合蛋白，对PS有很高的亲和性，并且可以与暴露于细胞外的PS相结合。利用这一原理，可以将AnnexinV标记荧光来识别早期的细胞凋亡。通常利用AnnexinV-FITC和碘化丙啶(P1)来区分凋亡和坏死细胞。PI
Tumor Targeting by a Carbohydrate Ligand-Mimicking Peptide
Annexin A1 (Anxa1) is a highly specific surface marker of tumor vasculature. We used peptide-displaying phage technology to identify a carbohydrate ligand-mimicking 7-mer peptide, IFLLWQR (IF7), which can target Anxa1 in tumor vasculature
。根据Ap峰的百分率可检测凋亡细胞数。在本组研究中PI法凋亡检出率为27.19%,显著低于TUNEL法及Annexin V/PI法。其检出率低的原因主要是凋亡早期尽管有DNA 裂点形成，但尚未出现DNA 片段的大量丢失，因此该法不能检出早期凋亡细胞。次要原因是发生于S期或G2 /M期的凋亡细胞，即使有DNA 含量降低，其实际含量也不低于二倍体细胞所含的DNA 。国内李莉报道采用与本文同一动物模型PI漏检率高达42.8%。此外PI法还存在错检，一部分坏死细胞碎片发生低荧光，位于二倍体峰前，造成PI法错检
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