- ￥1350 - 3000
- Proteintech
- 18244-1-AP
- 2026年05月21日
- WB, IHC, IF/ICC, IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human EIF2AK2 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IP, ELISA
- 靶点：
EIF2AK2, PKR
- 抗体英文名：
EIF2AK2, PKR Polyclonal antibody
- 抗体名：
EIF2AK2, PKR Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A549 cells, Jurkat cells, mouse thymus tissue, HepG2 cells, HeLa cells, rat heart tissue, K-562 cells, MCF-7 cells, SH-SY5Y cells, THP-1 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18244-1-AP targets EIF2AK2, PKR in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig, rabbit, goat |
| 免疫原 |
CatNo: Ag13010 Product name: Recombinant human EIF2AK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-373 aa of BC093676 Sequence: MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGT |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 2-alpha kinase 2 |
| 别名 | EIF2AK2, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2 |
| 计算分子量 | 551 aa, 62 kDa |
| 观测分子量 | 65-68 kDa |
| GenBank蛋白编号 | BC093676 |
| 基因名称 | EIF2AK2 |
| Gene ID (NCBI) | 5610 |
| RRID | AB_2246451 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P19525 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
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文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Cancer Cell p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.Authors - Alessandro Carugo |
| mouse | WB | Nat Neurosci Maternal immune activation in mice disrupts proteostasis in the fetal brain.Authors - Brian T Kalish |
| human | WB | Sci Adv Temporal control of the integrated stress response by a stochastic molecular switch.Authors - Philipp Klein |
| human | WB | Nat Commun NSs amyloid formation is associated with the virulence of Rift Valley fever virus in mice.Authors - Psylvia Léger |
| human | WB | J Immunother Cancer Loss of ADAR1 in macrophages in combination with interferon gamma suppresses tumor growth by remodeling the tumor microenvironmentAuthors - Weiwei Lin |
| human,mouse | WB | Int J Antimicrob Agents Pan-viral propagation blockade by inhibiting host cell PNPT1Authors - Shuang Qu |
亲和探针，通过基于活性的蛋白质组分析技术（ABPP）寻找抗炎的关键靶点。他们首先通过多学科交叉的手段，首次发现并验证 EIF2AK2（也称为 PKR）、eEF1A1、PRDX3 和 VPS4B 作为 BBR 的直接靶点，发挥协同抗炎作用。其中，BBR 通过离子键与 EIF2AK2 强结合，并通过 EIF2AK2 同时调控多种炎症通路，表明 EIF2AK2 是 BBR 抗炎作用的关键靶标。此外，BBR 可以巧妙地抑制 EIF2AK2 二聚体形成而不是直接抑制其酶活性，并选择性抑制 JNK、NF-κB
content gene Gpc-B1 and development of a high-throughput molecular marker. New Phytologist 169:753-763. Andrey A. Perelygin, Teri L. Lear, Andrey A. Zharkikh and Margo A. Brinton (2006) Comparative analysis of vertebrate EIF2AK2 (PKR) genes
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
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