- ￥1350 - 3000
- Proteintech
- 17564-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IF-P, IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human CD146, MCAM protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IF-P, IP, ELISA
- 靶点：
CD146/MCAM
- 抗体英文名：
CD146/MCAM Polyclonal antibody
- 抗体名：
CD146/MCAM Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | HeLa cells, A375 cells, mouse lung tissue, HUVEC cells, HepG2 cells |
| Positive IP detected in | HeLa cells, HepG2 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse spleen tissue |
| Positive IF/ICC detected in | HUVEC cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20000-1:80000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17564-1-AP targets CD146/MCAM in WB, IHC, IF/ICC, IF-P, IP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, Cell treatment |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, rabbit |
| 免疫原 |
CatNo: Ag11762 Product name: Recombinant human CD146, MCAM protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-350 aa of BC056418 Sequence: GEAEQPAPELVEVEVGSTALLKCGLSQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGATLALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPVNSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSGLYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSP |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | melanoma cell adhesion molecule |
| 别名 | CD146, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, MUC18 |
| 计算分子量 | 646 aa, 72 kDa |
| 观测分子量 | 113-120 kDa |
| GenBank蛋白编号 | BC056418 |
| 基因名称 | CD146 |
| Gene ID (NCBI) | 4162 |
| RRID | AB_2143373 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P43121 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | IHC | Biomaterials Macropore design of tissue engineering scaffolds regulates mesenchymal stem cell differentiation fate.Authors - W Benton Swanson |
| rat | IF | Adv Healthc Mater Extrafibrillarly Demineralized Dentin Matrix for Bone RegenerationAuthors - Xiaoyi Wu |
| human | WB | Oncogene The histone demethylase KDM3A, and its downstream target MCAM, promote Ewing Sarcoma cell migration and metastasis.Authors - M Sechler
|
| human | IF | Cell Prolif Dental pulp stem cells-based therapy for the oviduct injury via immunomodulation and angiogenesis in vivo.Authors - Lihua Luo |
| human | Stem Cell Res Ther Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axisAuthors - Zuping Wu | |
| rat | WB | J Proteome Res Comparative Proteomic Analysis of Primary Schwann Cells and a Spontaneously Immortalized Schwann Cell Line RSC 96: A Comprehensive Overview with a Focus on Cell Adhesion and Migration Related Proteins.Authors - Ji Yuhua Y
|
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