- ￥1350 - 3000
- Proteintech
- 15513-1-AP
- 2025年10月13日
- WB, IHC, IF-P, IF-Fro, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human cTnT protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF-P, IF-Fro, ELISA
- 靶点：
Cardiac Troponin T
- 抗体英文名：
Cardiac Troponin T Polyclonal antibody
- 抗体名：
Cardiac Troponin T Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | pig heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue, mouse heart tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF-Fro detected in | mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15513-1-AP targets Cardiac Troponin T in WB, IHC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, IF-Fro, ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, zebrafish |
| 免疫原 |
CatNo: Ag7716 Product name: Recombinant human cTnT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC002653 Sequence: MSDIEEVVEEYEEEEQEEAAVEEQEEAAEEDAEAEAETEETRAEEDEEEEEAKEAEDGPMEESKPKPRSFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQALIEAHFENRKKEEEELVSLKDRIERRRAERAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQSIYNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | troponin T type 2 (cardiac) |
| 别名 | cTnT, TNNT2, Troponin T, cardiac muscle |
| 计算分子量 | 36 kDa |
| 观测分子量 | 34-40 kDa |
| GenBank蛋白编号 | BC002653 |
| 基因名称 | Cardiac Troponin T |
| Gene ID (NCBI) | 7139 |
| RRID | AB_2206563 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P45379 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | IF | Nature Brahma safeguards canalization of cardiac mesoderm differentiation.Authors - Swetansu K Hota |
| pig | IF | Cell Stem Cell Generation of a humanized mesonephros in pigs from induced pluripotent stem cells via embryo complementationAuthors - Jiaowei Wang |
| human | IF | Sci Transl Med Precise genomic editing of pathogenic mutations in RBM20 rescues dilated cardiomyopathyAuthors - Takahiko Nishiyama |
| human | IF | ACS Nano Intrinsic Color Sensing System Allows for Real-Time Observable Functional Changes on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.Authors - Yiqi Gong |
| mouse | IF | Acta Pharm Sin B Hippo pathway-manipulating neutrophil-mimic hybrid nanoparticles for cardiac ischemic injury via modulation of local immunity and cardiac regenerationAuthors - Qiaozi Wang |
| mouse | IF | Biomaterials Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failureAuthors - Lingjun Tong |
Myofilament Protein Phosphorylation by PKC in Genetically Engineered Adult Cardiac Myocytes
in myocytes now makes it possible to investigate the relative phosphorylation of a given contractile protein as it resides within the intact myofilament. Contractile proteins, including troponin I (TnI), troponin T (TnT), myosin light chain 2 (MLC
Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
Bovine Fibrinogen Sigma F8630 Construct Bovine Thrombin Sigma T7513
-rich region] of the rat cardiac troponin T promoter. In GMSA, the specificity of the binding of trans-acting factor to labeled DNA probe should be verified by the addition of unlabeled probe in the reaction mixture. The migratory property of DNA-protein
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