- ￥1350 - 3000
- Proteintech
- 14395-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA
- Rabbit
- human, mouse, rat
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human ACTA2 protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human, mouse, rat
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA
- 靶点：
Alpha smooth muscle actin
- 抗体英文名：
Alpha smooth muscle actin Polyclonal antibody
- 抗体名：
Alpha smooth muscle actin Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | mouse colon tissue, human placenta tissue, A549 cells, HUVEC cells, rat colon tissue, mouse heart tissue, rat heart tissue, mouse liver tissue, mouse kidney tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, human heart tissue, human colon tissue, human hysteromyoma tissue, stromal tumor tissue, human appendicitis tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue, mouse small intestine tissue |
| Positive IF-Fro detected in | mouse colon tissue |
| Positive IF/ICC detected in | C2C12 cells, H9C2 cells |
| Positive FC (Intra) detected in | C2C12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| Immunofluorescence (IF)-P | IF-P : 1:1000-1:4000 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:800-1:3200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14395-1-AP targets Alpha smooth muscle actin in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, ELISA, competitive binding assay applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA |
| 文献引用应用 | WB, IHC, IF, CoIP, competitive binding assay |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, rabbit, canine, monkey, zebrafish, bovine, sheep |
| 免疫原 |
CatNo: Ag5886 Product name: Recombinant human ACTA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-377 aa of BC017554 Sequence: MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | actin, alpha 2, smooth muscle, aorta |
| 别名 | ACTA2, Alpha SMA, a-SMA, smooth muscle actin, a SMA |
| 计算分子量 | 377 aa, 42 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC017554 |
| 基因名称 | Alpha smooth muscle actin |
| Gene ID (NCBI) | 59 |
| RRID | AB_2223009 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62736 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.
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- 作者
- 内容
- 询问日期
文献和实验| Species | Application | Title |
|---|---|---|
| human | IHC | Nat Genet Spatially resolved transcriptomic analysis of the adult human prostateAuthors - Junyi Hu |
| rat | IF | Adv Mater Exoskeleton Partial-Coated Stem Cells for Infarcted Myocardium RestoringAuthors - Huihui He |
| mouse | WB | Cell Metab Augmentation of scleral glycolysis promotes myopia through histone lactylationAuthors - Xiaolei Lin |
| mouse,human | IF | Cell Metab Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancerAuthors - Yu Jiang |
| mouse | IF | Mil Med Res Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver diseaseAuthors - Guang-Hui Deng |
| mouse | WB | Nat Cell Biol LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.Authors - Pingping Zhu |
Isolation and Culture of Human Airway Smooth Muscle Cells
doublings and express smooth muscle cell-specific markers such as myosin heavy chain, myosin light chain kinase, α-actin, caldesmon, and SM22α . In addition, the synthetic function of these cells appears to be comparable to those studied in vivo
Protein purification: skeletal muscle myosin
Overview SKELETAL MUSCLE MYOSIN A two-headed hexomeric actin-activated ATPase consisting of two heavy chains of ~200 kDa and four light chains of ~20kDa. The tail portion
muscle cells in culture.Physiol Rev 1979 59（1）:1-61. 2.Chambers P,Neal DE,Gillespie JI.Ca2+ signalling in cultured smooth muscle cells from human bladder .1996 Exp Physiol 81 (4):553-564. 3.Korkmaz M, Guvenc BH, Bilir A et al. Isolation and culture
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