- ￥1350 - 3000
- Proteintech
- 13196-1-AP
- 2025年10月13日
- WB, IP, ELISA
- Rabbit
- human
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human AXL protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IP, ELISA
- 靶点：
AXL
- 抗体英文名：
AXL Polyclonal antibody
- 抗体名：
AXL Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | DU 145 cells, HeLa cells, HepG2 cells, human liver tissue, HT-1080 cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13196-1-AP targets AXL in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag3898 Product name: Recombinant human AXL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 545-894 aa of BC032229 Sequence: GEFGAVMEGQLNQDDSILKVAVKTMKIAICTRSELEDFLSEAVCMKEFDHPNVMRLIGVCFQGSERESFPAPVVILPFMKHGDLHSFLLYSRLGDQPVYLPTQMLVKFMADIASGMEYLSTKRFIHRDLAARNCMLNENMSVCVADFGLSKKIYNGDYYRQGRIAKMPVKWIAIESLADRVYTSKSDVWSFGVTMWEIATRGQTPYPGVENSEIYDYLRRGNRLKQPADCLDGLYALMSRCWELNPQDRPSFTELREDLENTLKALPPAQEPDEILYVNMDEGGGYPEPPGAAGGADPPTQPDPKDSCSCLTAAEVHPAGRYVLCPSTTPSPAQPADRGSPAAPGQEDGA |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | AXL receptor tyrosine kinase |
| 别名 | EC:2.7.10.1, Tyrosine-protein kinase receptor UFO, UFO |
| 计算分子量 | 894 aa, 98 kDa |
| 观测分子量 | 140 kDa |
| GenBank蛋白编号 | BC032229 |
| 基因名称 | AXL |
| Gene ID (NCBI) | 558 |
| RRID | AB_10642006 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30530 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验| Species | Application | Title |
|---|---|---|
| human | WB | Int J Cancer Therapeutic Activity of DCC-2036, a Novel Tyrosine Kinase Inhibitor, against Triple-Negative Breast Cancer Patient-Derived Xenografts by Targeting AXL/MET.Authors - Yingying Shen |
| rat | WB | Int J Mol Sci Embryonic-Derived Myb- Macrophages Enhance Bacterial Clearance and Improve Survival in Rat Sepsis.Authors - Mirjana Jerkic |
| human | WB | Int J Mol Sci Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.Authors - Elisa De Tomi |
| WB,IHC | Int Immunopharmacol C1632 protects against LPS-induced acute lung injury by regulating AXL-mediated MAPK/NF-κB signaling pathwayAuthors - Yanan Mei | |
Bioorg Chem Non-volatile acylphloroglucinol components from Eucalyptus robusta inhibit Zika virus by impairing RdRp activity of NS5.Authors - Zhai-Wen Yao | ||
| human | WB,IHC,IF | Cancer Gene Ther Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.Authors - Minhai Dong |
CANDOR BIOSCIENCE: 关于ELISA板稳定性的技术探讨与比较
物稳定剂，例如CANDOR的Antibody Stabilizer、HRP-Protector™和AP-Protector®，可以大大提高对照品和检测抗体-结合物的稳定性。本文以ELISAs为例，重点讨论固相稳定、表面包被捕获分子的活性维持的机理和解决方案。此外，本文概述的原理和解决方案也可以应用于其他包被表面，如聚苯乙烯或玻璃表面的实验应用，如侧向层析、亲和柱、基于微球的分析、免疫PCR或蛋白质分析等。 包被的捕获分子如抗体，或在抗原下降分析中分离的病毒蛋白，是可靠免疫分析的核心组成
因此，综合来说，EarthOx的GST标签抗体 （货号#E022040）是一款同时具有高特异性、高灵敏度、多应用类型以及性价比的GST标签抗体。同时，我们也提供其他品牌的GST标签抗体，如Proteintech的GST多克隆抗体 ――GST PolyClonal Antibody（货号#10000-0-AP）以及Abcam、Santa等多种GST标签抗体产品。更多GST标签抗体以及其他如His、HA、Myc、GFP、Flag等其他标签抗体，请电话垂询。美国EarthOx品牌是一家提供高性价比免疫学和细胞
实验，请根据说明书列出已验证的种属来选择适合你实验的抗体。 （4）样本蛋白的结构性质。了解样本蛋白的结构性质有助于选择最合适的抗体，待测样本蛋白的结构域和样本在提取和处理过程中是否会变性，蛋白空间构象的改变，会影响抗体的免疫亲和反应。 （5）单多克隆抗体的选择。市面上的抗体还是以多克隆抗体为主，一般单克隆抗体特异性强，但亲和力相对小，检测抗原灵敏度相对就低；而多克隆抗体特异性稍弱，但抗体的亲和力强，灵敏度高，但易出现非特异性染色（可以通过封闭等避免
技术资料暂无技术资料 索取技术资料
