- ￥1350 - 3000
- Proteintech
- 12216-1-AP
- 2025年10月13日
- WB, IHC, IF/ICC, FC (Intra), ELISA
- Rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant human Cathepsin B protein
- 亚型：
IgG
- 保存条件：
Store at -20°C. Stable for one year after shipment.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
human
- 保质期：
详见产品包装
- 库存：
充足
- 宿主：
Rabbit
- 应用范围：
WB, IHC, IF/ICC, FC (Intra), ELISA
- 靶点：
Cathepsin B
- 抗体英文名：
Cathepsin B Polyclonal antibody
- 抗体名：
Cathepsin B Polyclonal antibody
- 规格：
50ul/100ul/150ul
| 规格： | 50ul | 产品价格： | ￥1350.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥2200.0 |
| 规格： | 150ul | 产品价格： | ￥3000.0 |
经过测试的应用
| Positive WB detected in | A375 cells, HepG2 cells, HT-1080 cells |
| Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12216-1-AP targets Cathepsin B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, pig, bovine, frog |
| 免疫原 |
CatNo: Ag2898 Product name: Recombinant human Cathepsin B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-339 aa of BC010240 Sequence: MWQLWASLCCLLVLANARSRPSFHPVSDELVNYVNKRNTTWQAGHNFYNVDMGYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQYWEKI |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cathepsin B |
| 别名 | CTSB, APP secretase, APPS, Cat B, Cathepsin B heavy chain |
| 计算分子量 | 339 aa, 38 kDa |
| 观测分子量 | 25 kDa, 31 kDa, 43 kDa |
| GenBank蛋白编号 | BC010240 |
| 基因名称 | Cathepsin B |
| Gene ID (NCBI) | 1508 |
| RRID | AB_2086929 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07858 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 43 kDa form of pro-CTSB, 31 kDa and 25 kDa mature forms in mouse brain by western blot(PMID:20616152).
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文献和实验| Species | Application | Title |
|---|---|---|
| mouse | WB,IF | Nature Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.Authors - Jiasheng Zhang |
| rat | WB | Cell Death Differ NLRX1 mediated impaired microglial phagocytosis of NETs in cerebral ischemia and reperfusion injuryAuthors - Jialing Peng |
| human | WB | Autophagy Atractylenolide I inhibits angiogenesis and reverses sunitinib resistance in clear cell renal cell carcinoma through ATP6V0D2-mediated autophagic degradation of EPAS1/HIF2αAuthors - Qinyu Li |
| human | IF | Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degenerationAuthors - Huaizhen Liang |
| mouse | WB,IHC | Aging Cell Growth differentiation factor 11 accelerates liver senescence through the inhibition of autophagyAuthors - Jian Sun |
| human | WB | Redox Biol Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.Authors - Shumin Ouyang |
大鼠 apelin (AP) 酶联免疫分析（ ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定大鼠血清，血浆及相关液体样本中 apelin (AP) 的 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中 大鼠 apelin (AP) 水平。用纯化的 apelin (AP) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 apelin (AP
人 apelin 1 2 (AP1 2 ) 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 apelin 12(AP12) 的 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 人 apelin 1 2 (AP1 2 ) 水平。用纯化的 抗- apelin 1 2 (AP1 2 ) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中
组织蛋白酶 cathepsin 在各种动物组织的细胞内（特别是溶酶体部分）发现的一类蛋白酶。此名源自希腊语的“消化”，为威尔斯达特（ R． Willstā atter， 1929）命名的。来源于同一种类组织细胞的也是由组织蛋白酶 A． B． C． D． E等多种酶组成。可以根据所作用的代表性底物的种类进行分类，但似乎各自还有数种同工酶。组织蛋白酶 A的代表性底物是 N-苄氧基羰基 -L-谷酰胺 -L-苯丙氨酸，最适 pH约 5.5（ 3— 6），可能是一种羧肽酶，可为二异丙基磷氟酸
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